Rosa J, Mehta A, Filipe M I
Department of Histopathology, Portuguese Institute of Oncology, Lisbon.
Histopathology. 1990 Mar;16(3):265-9. doi: 10.1111/j.1365-2559.1990.tb01113.x.
A silver technique for nucleolar organizer regions (AgNOR) was applied to sections from 156 gastric biopsies and gastrectomy specimens. These included normal controls, normal gastric mucosa from carcinoma-bearing stomachs, intestinal metaplasia types I and III, dysplasia and carcinoma. AgNOR counts gradually increased from normal, through intestinal metaplasia, to carcinoma. This finding supports the chronic atrophic gastritis-intestinal metaplasia-dysplasia-carcinoma sequence concept for gastric carcinogenesis. Normal gastric mucosa was different from all lesions, including normal mucosa from carcinoma-bearing stomachs. Significantly higher AgNOR counts were observed in tumours compared to all other lesions except dysplasia. Dysplasia differed from intestinal metaplasia type I but not from type III. Eighty-five per cent of metaplasia cases overlapped with carcinoma and 19% with normal controls. The spread of AgNOR values in intestinal metaplasia reinforces the concept that this lesion is a heterogeneous entity reflecting a dynamic and continuous process. The AgNOR technique may contribute to the assessment of the stage of evolution of 'borderline' lesions.
一种用于核仁组成区（AgNOR）的银染技术应用于156例胃活检和胃切除标本的切片。这些标本包括正常对照、来自患癌胃的正常胃黏膜、I型和III型肠化生、发育异常和癌。AgNOR计数从正常组织开始，经肠化生至癌逐渐增加。这一发现支持了胃癌发生的慢性萎缩性胃炎-肠化生-发育异常-癌序列概念。正常胃黏膜与所有病变不同，包括来自患癌胃的正常黏膜。与除发育异常外的所有其他病变相比，肿瘤中的AgNOR计数显著更高。发育异常与I型肠化生不同，但与III型无差异。85%的化生病例与癌重叠，19%与正常对照重叠。AgNOR值在肠化生中的分布强化了这一概念，即该病变是一个反映动态连续过程的异质性实体。AgNOR技术可能有助于评估“临界”病变的演变阶段。
