Rapid technique for cryopreservation of Echinococcus multilocularis metacestodes.
作者信息
Bretagne S, Beaujean F, Uner A, Bresson-Hadni S, Liance M, Houin R
机构信息
Laboratoire de Parasitologie, Faculté de Médecine, Creteil, France.
出版信息
Int J Parasitol. 1990 Apr;20(2):265-7. doi: 10.1016/0020-7519(90)90111-y.
PMID:2332285
Abstract
Cysts of E. multilocularis were minced to prepare a crude homogenate and after addition of glycerol at a final concentration of 10%, cryopreservation was performed at a rate of 1 degree C min-1 in a controlled-rate freezer. The aliquots were subsequently stored in liquid nitrogen. All 22 isolates tested were successfully cryopreserved and their viability maintained.
摘要
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