评估用于从浸渍全血的滤纸圆盘检测1型人类免疫缺陷病毒抗体的酶联免疫分析系统。
Evaluation of enzyme-linked immunoassay systems for detection of human immunodeficiency virus type 1 antibody from filter paper disks impregnated with whole blood.
作者信息
Beebe J L, Briggs L C
机构信息
Laboratory Division, Colorado Department of Health, Denver 80220.
出版信息
J Clin Microbiol. 1990 Apr;28(4):808-10. doi: 10.1128/jcm.28.4.808-810.1990.
Abstract
Five commercial enzyme-linked immunoassay systems for the detection of human immunodeficiency virus type 1 antibody from filter paper disks impregnated with whole blood were evaluated for technical and operational performance. All five systems performed adequately in the technical challenges posed, with specificities in excess of 99% for 1,020 specimens. In a serial dilution sensitivity challenge, all of the kits were able to detect specific antibody within one dilution of a Western blot (immunoblot) standard, except for a Du Pont Co. kit, which detected antibody within two dilutions of the standard. The Du Pont assay showed the least variation in control values between test runs and between lots. All of the systems produced acceptable results, but their operational parameters differed significantly.
摘要
对五种用于从浸渍全血的滤纸圆盘检测1型人类免疫缺陷病毒抗体的商业酶联免疫分析系统进行了技术和操作性能评估。在面临的技术挑战中，所有这五种系统表现良好，对1020份标本的特异性均超过99%。在连续稀释敏感性挑战中，除杜邦公司的试剂盒在标准品的两次稀释内检测到抗体外，所有试剂盒都能在蛋白质印迹法（免疫印迹）标准品的一次稀释内检测到特异性抗体。杜邦分析在各测试批次之间以及各批次之间的对照值变化最小。所有系统都产生了可接受的结果，但它们的操作参数有显著差异。
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