Klug S J, Famulok M
Institut für Molekulare Biochemie, Genzentrum der Ludwig-Maximillian-Universität Müchen, Germany.
Mol Biol Rep. 1994;20(2):97-107. doi: 10.1007/BF00996358.
In vitro selection, or SELEX, is a technique that allows the simultaneous screening of highly diverse pools of different RNA or DNA (dsDNA or ssDNA) molecules for a particular feature. Different examples from a great variety of applications of in vitro selection experiments are described and a detailed overview of the method and its variations will be given. Some especially conclusive in vitro selection experiments are discussed in detail to illustrate the potential power and diversity of this method. Potential restrictions of the methods and possible ways to overcome them are pointed out.
体外筛选，即指数富集的配体系统进化（SELEX），是一种能够同时针对特定特征对高度多样化的不同RNA或DNA（双链DNA或单链DNA）分子库进行筛选的技术。文中描述了体外筛选实验在众多应用中的不同实例，并将对该方法及其变体进行详细概述。还将详细讨论一些特别有说服力的体外筛选实验，以说明该方法的潜在威力和多样性。同时指出了该方法的潜在限制以及克服这些限制的可能途径。
