皮肤中RNA干扰介导的基因功能分析。
RNAi-mediated gene function analysis in skin.
作者信息
Beronja Slobodan, Fuchs Elaine
机构信息
Laboratory of Mammalian Cell Biology & Development, Howard Hughes Medical Institute, The Rockefeller University, New York, NY, USA.
出版信息
Methods Mol Biol. 2013;961:351-61. doi: 10.1007/978-1-62703-227-8_23.
Abstract
We have recently developed a method for RNAi-mediated gene function analysis in skin (Beronja et al., Nat Med 16:821-827, 2010). It employs ultrasound-guided in utero microinjections of lentivirus into the amniotic cavity of embryonic day 9 mice, which result in rapid, efficient, and stable transduction into mouse skin. Our technique greatly extends the available molecular and genetic toolbox for comprehensive functional examination of outstanding problems in epidermal biology. In its simplest form, as a single-gene function analysis via shRNA-mediated gene knockdown, our technique requires no animal mating and may need as little as only a few days between manipulation and phenotypic analysis.
摘要
我们最近开发了一种用于皮肤中RNAi介导的基因功能分析的方法（Beronja等人，《自然医学》16:821 - 827，2010年）。该方法采用超声引导下在胚胎第9天的小鼠羊膜腔内进行慢病毒子宫内显微注射，从而快速、高效且稳定地转导至小鼠皮肤。我们的技术极大地扩展了可用于全面功能研究表皮生物学突出问题的分子和遗传工具箱。以其最简单的形式，作为通过shRNA介导的基因敲低进行的单基因功能分析，我们的技术无需动物交配，从操作到表型分析可能只需短短几天时间。
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