亲环素 A 依赖性限制对广泛细胞系中 N121K 突变型人类免疫缺陷病毒 1 衣壳的作用。
Cyclophilin A-dependent restriction to capsid N121K mutant human immunodeficiency virus type 1 in a broad range of cell lines.
机构信息
AIDS Research Center, National Institute of Infectious Diseases, Shinjuku-ku, Tokyo, Japan.
出版信息
J Virol. 2013 Apr;87(7):4086-90. doi: 10.1128/JVI.01319-12. Epub 2013 Jan 16.
Abstract
Human immunodeficiency virus type 1 (HIV-1) infection of most human cells is dependent on cyclophilin A (CypA); however, the opposite phenomenon, known as CypA-dependent inhibition, is also observed in the combination of some capsid (CA) mutations and cell lines. Here, we identified a CA N121K mutant whose infection of 293T, Jurkat, and HeLa cells was impaired by CypA. The N121K mutant could be a useful tool for analyzing the mechanisms underlying CypA-dependent restriction.
摘要
人类免疫缺陷病毒 1 型（HIV-1）对大多数人类细胞的感染依赖于亲环素 A（CypA）；然而，在一些衣壳（CA）突变和细胞系的组合中也观察到相反的现象，即 CypA 依赖性抑制。在这里，我们鉴定出一种 CA N121K 突变体，其对 293T、Jurkat 和 HeLa 细胞的感染被 CypA 所抑制。N121K 突变体可作为分析 CypA 依赖性限制机制的有用工具。
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