Tang Xinhui, Gao Jing, Wang Yanping, Fan Yi-Mei, Xu Li-Zhi, Zhao Xiao-Ning, Xu Qiang, Qian Zhong Ming
Institute of Materia Medica, School of Medicine and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, PR China.
J Nutr Biochem. 2006 Mar;17(3):177-82. doi: 10.1016/j.jnutbio.2005.06.008. Epub 2005 Aug 10.
The protective effects of chloroform extracts of Terminalia catappa L. leaves (TCCE) on carbon tetrachloride (CCl4)-induced liver damage and the possible mechanisms involved in the protection were investigated in mice. We found that increases in the activity of serum aspartate aminotransferase and alanine aminotransferase and the level of liver lipid peroxidation (2.0-fold, 5.7-fold and 2.8-fold) induced by CCl4 were significantly inhibited by oral pretreatment with 20, 50 or 100 mg/kg of TCCE. Morphological observation further confirmed the hepatoprotective effects of TCCE. In addition, the disruption of mitochondrial membrane potential (14.8%), intramitochondrial Ca2+ overload (2.1-fold) and suppression of mitochondrial Ca2+-ATPase activity (42.0%) in the liver of CCl4-insulted mice were effectively prevented by pretreatment with TCCE. It can be concluded that TCCE have protective activities against liver mitochondrial damage induced by CCl4, which suggests a new mechanism of the hepatoprotective effects of TCCE.
研究了榄仁树叶氯仿提取物（TCCE）对四氯化碳（CCl4）诱导的小鼠肝损伤的保护作用及其可能的保护机制。我们发现，用20、50或100mg/kg的TCCE进行口服预处理可显著抑制CCl4诱导的血清天冬氨酸氨基转移酶和丙氨酸氨基转移酶活性增加以及肝脂质过氧化水平（分别增加2.0倍、5.7倍和2.8倍）。形态学观察进一步证实了TCCE的肝保护作用。此外，TCCE预处理可有效预防CCl4损伤小鼠肝脏中线粒体膜电位的破坏（14.8%）、线粒体内Ca2+超载（2.1倍）以及线粒体Ca2+-ATP酶活性的抑制（42.0%）。可以得出结论，TCCE对CCl4诱导的肝线粒体损伤具有保护活性，这提示了TCCE肝保护作用的一种新机制。
