Yeom Su-Cheong, Cho Seong-Yong, Park Chung-Gyu, Lee Wang-Jae
Bio-Medical Center for Animal Resource and Development, Bio-max institute, Seoul National University, Seoul, Korea. ; Xenotransplantation Research Center, Seoul National University Hospital, Seoul, Korea.
Lab Anim Res. 2012 Dec;28(4):245-53. doi: 10.5625/lar.2012.28.4.245. Epub 2012 Dec 26.
We analyzed 72 items related to serum biochemistry and hematology in 85 specific pathogen-free (SPF) Seoul National University (SNU) miniature pigs aged 1- to 36-months which originated from a Minnesota miniature pig. Almost all examined items were similar between male and female pigs. However, some items such as Cr level, B/C ratio, C.R.F, LDH, LAP and T4 were significantly different between male and female pigs (P<0.05). Thirty four examined items showed age-related changes, and the significant changes were observed in animals less than six months old. The values for BUN, K, uric acid, Ca, Ca(++), and Pi were significantly higher in pigs younger than six months of age, which might reflect poor kidney function in young pigs. Additionally, TIBC, UIBC and RDW were significantly higher in young pigs, and RBC, Hb, HCT, MCHC and MCV were significantly lower in young pigs, thus indicating a similar physiology of iron deficiency anemia. These age-related specific phenotypes seemed to be normal, but it should be considered in the long-term experiment using the young pigs. In conclusion, in this study, we defined the normal reference intervals for SPF SNU miniature pigs, and we also determined that there are some physiological differences between the pig genders and ages. This study provides fundamental data for use in experiments involving SPF SNU miniature pigs.
我们分析了85只1至36月龄、源自明尼苏达小型猪的首尔国立大学无特定病原体（SPF）小型猪的72项血清生化和血液学指标。几乎所有检测指标在雄性和雌性猪之间都相似。然而，一些指标，如肌酐水平、B/C比值、促肾上腺皮质激素释放因子、乳酸脱氢酶、碱性磷酸酶和甲状腺素，在雄性和雌性猪之间存在显著差异（P<0.05）。34项检测指标显示出与年龄相关的变化，且在6月龄以下的动物中观察到显著变化。6月龄以下仔猪的尿素氮、钾、尿酸、钙、钙离子和无机磷的值显著更高，这可能反映了仔猪肾功能较差。此外，6月龄以下仔猪的总铁结合力、未饱和铁结合力和红细胞分布宽度显著更高，而红细胞、血红蛋白、血细胞比容、平均红细胞血红蛋白浓度和平均红细胞体积显著更低，从而表明存在缺铁性贫血的相似生理状态。这些与年龄相关的特定表型看似正常，但在使用仔猪的长期实验中应予以考虑。总之，在本研究中，我们定义了SPF首尔国立大学小型猪的正常参考区间，并且还确定了猪的性别和年龄之间存在一些生理差异。本研究为涉及SPF首尔国立大学小型猪的实验提供了基础数据。
