Department of Biomedical Imaging and Radiological Sciences, School of Biomedical Science and Engineering, National Yang-Ming University, Taipei, Taiwan.
PLoS One. 2013;8(1):e54034. doi: 10.1371/journal.pone.0054034. Epub 2013 Jan 10.
This study demonstrates the feasibility of using focused ultrasound (FUS) to modulate glomerular ultrafiltration by renal artery sonication and determine if protein-creatinine ratios are estimated through vascular parameters. All animal experiments were approved by our Animal Care and Use Committee. The renal arteries of Sprague-Dawley rats were surgically exposed and sonicated at various acoustic power levels using a FUS transducer with a resonant frequency of 1 MHz. The mean peak systolic velocity (PSV) of the blood flow was measured by Doppler ultrasound imaging. Urinary protein-creatinine ratios were calculated during the experiments. Histological examination of renal arteries and whole kidneys was performed. The PSV, pulsatility index, and resistance index of blood flow significantly increased in the arteries after FUS sonication without microbubbles (p<0.05). The change in normalized protein-creatinine ratios significantly increased with increasing acoustic power, but such was not observed when microbubbles were administered. Furthermore, no histological changes were observed in the hematoxylin- and eosin-stained sections. Glomerular ultrafiltration is regulated temporarily by renal artery sonication without microbubbles. Monitoring vascular parameters are useful in estimating the normalized change in protein-creatinine ratios.
本研究证明了使用聚焦超声（FUS）通过肾动脉超声处理调节肾小球超滤的可行性，并确定血管参数是否可以估计蛋白-肌酐比值。所有动物实验均获得了我们的动物护理和使用委员会的批准。通过使用谐振频率为 1 MHz 的 FUS 换能器，对 Sprague-Dawley 大鼠的肾动脉进行了手术暴露，并在不同声功率水平下进行了超声处理。通过多普勒超声成像测量血流的平均峰值收缩速度（PSV）。在实验过程中计算尿蛋白-肌酐比值。对肾动脉和整个肾脏进行组织学检查。在没有微泡的情况下，FUS 超声处理后血液的 PSV、脉动指数和阻力指数显著增加（p<0.05）。归一化蛋白-肌酐比值的变化随声功率的增加而显著增加，但当给予微泡时则没有观察到这种情况。此外，苏木精和伊红染色切片中未观察到组织学变化。肾小球超滤通过无微泡的肾动脉超声处理暂时调节。监测血管参数有助于估计蛋白-肌酐比值的归一化变化。
