乳腺乳头瘤和乳头状癌中细胞周期蛋白 D1、细胞角蛋白 5/6 和细胞角蛋白 8/18 表达的分析。
An analysis of cyclin D1, cytokeratin 5/6 and cytokeratin 8/18 expression in breast papillomas and papillary carcinomas.
机构信息
Department of Pathology, The 2nd affiliated hospital of Harbin Medical University, Xuefu Road 246, Harbin, Nanggang District, China.
出版信息
Diagn Pathol. 2013 Jan 18;8:8. doi: 10.1186/1746-1596-8-8.
BACKGROUND
To evaluate the expression levels of cyclin D1 in breast papillomas and papillary carcinomas, and to analyze the types of cells that co-express cyclin D1 with cytokeratin 5/6 (CK 5/6) or with cytokeratin 8/18(CK 8/18).
METHODS
Fifty-nine cases of papillary lesions including 36 papillomas and 23 intracystic papillary carcinomas were examined. Cyclin D1, CK 5/6 and CK 8/18 expression levels were evaluated by double immunostaining.
RESULTS
Cyclin D1 is highly expressed in papillary carcinomas (27.54% ± 15.43%) compared with papillomas (8.81% ± 8.41%, p < 0.01). Cyclin D1 is predominantly expressed in cytokeratin 8/18- expressing cells, rather than in cytokeratin 5/6-expressing cells, regardless of the type of lesion. In papillomas, cyclin D1 exhibited a mean 11.42% (11.42% ± 10.17%) co-expression rate with cytokeratin 8/18 compared with a mean 2.50% (2.50% ± 3.24%) co-expression rate with cytokeratin 5/6 (p < 0.01). In papillary carcinomas, cyclin D1 exhibited a mean 34.74% (34.74% ± 16.32%) co-expression rate with cytokeratin 8/18 compared with a co-expression rate of 0.70% (0.70% ± 0.93%) with cytokeratin 5/6 (p < 0.01).
CONCLUSIONS
The increase in cyclin D1 suggests an association of cyclin D1 staining with papillary carcinomas. Although cyclin D1 is an effective marker for the differential diagnosis of other papillary lesions, it cannot be used to distinguish between papilloma and papillary carcinoma lesions because its expression occurs in both lesions. Our results show that cyclin D1 and CK 5/6 staining could be used in concert to distinguish between the diagnosis of papilloma (cyclin D1 < 4.20%, CK 5/6 positive) or papillary carcinoma (cyclin D1 > 37.00%, CK 5/6 negative). In addition, our data suggest that cyclin D1 is expressed only in the cancer stem or progenitor cells that co-immunostained with CK 8/18 in papillary carcinomas, and predominantly with CK 8/18 in the papillomas.
VIRTUAL SLIDES
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背景
评估细胞周期蛋白 D1 在乳腺乳头瘤和乳突状癌中的表达水平，并分析与细胞角蛋白 5/6（CK5/6）或细胞角蛋白 8/18（CK8/18）共同表达的细胞类型。
方法
对 36 例乳头瘤和 23 例囊内乳突状癌共 59 例乳头状病变进行检查。通过双重免疫染色评估细胞周期蛋白 D1、CK5/6 和 CK8/18 的表达水平。
结果
与乳头瘤（8.81%±8.41%）相比，乳突状癌中细胞周期蛋白 D1 的表达水平（27.54%±15.43%）明显升高（p<0.01）。无论病变类型如何，细胞周期蛋白 D1 主要表达在细胞角蛋白 8/18 阳性细胞中，而不是在细胞角蛋白 5/6 阳性细胞中。在乳头瘤中，细胞周期蛋白 D1 与细胞角蛋白 8/18 的共同表达率平均为 11.42%（11.42%±10.17%），而与细胞角蛋白 5/6 的共同表达率平均为 2.50%（2.50%±3.24%）（p<0.01）。在乳突状癌中，细胞周期蛋白 D1 与细胞角蛋白 8/18 的共同表达率平均为 34.74%（34.74%±16.32%），而与细胞角蛋白 5/6 的共同表达率为 0.70%（0.70%±0.93%）（p<0.01）。
结论
细胞周期蛋白 D1 的增加提示细胞周期蛋白 D1 染色与乳突状癌有关。虽然细胞周期蛋白 D1 是鉴别其他乳头状病变的有效标志物，但不能用于区分乳头瘤和乳突状癌病变，因为两种病变均有其表达。我们的结果表明，细胞周期蛋白 D1 和 CK5/6 染色可以协同用于区分乳头瘤（细胞周期蛋白 D1<4.20%，CK5/6 阳性）或乳突状癌（细胞周期蛋白 D1>37.00%，CK5/6 阴性）的诊断。此外，我们的数据表明，在乳突状癌中，细胞周期蛋白 D1 仅在与 CK8/18 共免疫染色的癌症干细胞或祖细胞中表达，而在乳头瘤中主要与 CK8/18 表达。
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