Institute for Organic Chemistry, University of Stuttgart, 70569 Stuttgart, Germany.
J Org Chem. 2013 Feb 1;78(3):793-9. doi: 10.1021/jo3025779. Epub 2013 Jan 17.
Chemical primer extension is the enzyme-free incorporation of nucleotides at the end of an oligonucleotide, directed by a template. The reaction mimics the copying of sequences during replication but relies on recognition and reactivity of nucleic acids alone. Copying is low-yielding, particularly for long RNA. Hydrolysis of active esters and inhibition through hydrolysis products have been identified as factors that prevent high yields, and approaches to overcoming them have culminated in successful template-directed solid-phase syntheses for RNA and phosphoramidate DNA.
化学引物延伸是指在模板的指导下，在寡核苷酸的末端无酶掺入核苷酸。该反应模拟了复制过程中序列的复制，但仅依赖于核酸的识别和反应性。对于长 RNA，复制的产量很低。已确定活性酯的水解以及水解产物的抑制是防止高产率的因素，并且克服这些因素的方法最终导致了 RNA 和磷酰胺 DNA 的成功的模板指导固相合成。
