National Center for Soybean Improvement, National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, China.
Planta. 2013 May;237(5):1213-25. doi: 10.1007/s00425-012-1835-3. Epub 2013 Jan 18.
MicroRNAs (miRNA) are a class of non-coding RNAs that have important gene regulatory roles in various organisms. However, the miRNAs involved in soybean's response to soybean mosaic virus (SMV) are unknown. To identify novel miRNAs and biotic-stress regulated small RNAs that are involved in soybean's response to SMV, two small RNA libraries were constructed from mock-inoculated and SMV-infected soybean leaves and sequenced. This led to the discovery of 179 miRNAs, representing 52 families, among which five miRNAs belonging to three families were novel miRNAs in soybean. A large proportion (71.5 %) of miRNAs arose from segmental duplication, similar to the process that drives the evolution of protein-coding genes. In addition, we predicted 346 potential targets of these identified miRNAs, and verified 12 targets by modified 5'-RACE analysis. Finally, three miRNAs (miR160, miR393 and miR1510) that are involved in plant resistance were observed to respond to SMV infection. The interaction between miRNAs and resistance-related genes provides a novel mechanism for pathogens to evade host recognition.
MicroRNAs (miRNA) 是一类非编码 RNA，在各种生物体中具有重要的基因调控作用。然而，参与大豆对大豆花叶病毒 (SMV) 反应的 miRNA 尚不清楚。为了鉴定参与大豆对 SMV 反应的新 miRNA 和生物胁迫调节的小 RNA，我们从模拟接种和 SMV 感染的大豆叶片中构建了两个小 RNA 文库并进行了测序。这导致发现了 179 个 miRNA，代表 52 个家族，其中 5 个属于三个家族的 miRNA 是大豆中的新 miRNA。大部分 (71.5%) 的 miRNA 来源于片段重复，类似于驱动蛋白质编码基因进化的过程。此外，我们预测了这些鉴定的 miRNA 的 346 个潜在靶标，并通过改良的 5'-RACE 分析验证了 12 个靶标。最后，观察到三个参与植物抗性的 miRNA (miR160、miR393 和 miR1510) 对 SMV 感染有反应。miRNA 与抗性相关基因之间的相互作用为病原体逃避宿主识别提供了一种新机制。
