无标记电化学检测利用无细胞酵母-scFv 探针检测溶组织内阿米巴抗原。
Label-free electrochemical detection of an Entamoeba histolytica antigen using cell-free yeast-scFv probes.
机构信息
Centre for Biomarker Research and Development, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD 4072, Australia.
出版信息
Chem Commun (Camb). 2013 Feb 21;49(15):1551-3. doi: 10.1039/c2cc38882k.
Abstract
Inexpensive, simple and quick detection of pathogen antigens in human samples is a key global health objective. Limiting factors include the cost and complexity of diagnostic tests that utilize antibody probes. Herein, we present a method for label-free electrochemical detection of a protein from the enteric pathogen Entamoeba histolytica using cell-free yeast-embedded antibody-like fragments (yeast-scFv) as novel affinity reagents.
摘要
在人体样本中快速、简便、廉价地检测病原体抗原是一个全球性的健康目标。但目前的诊断检测方法受到成本和复杂性的限制，这些方法通常使用抗体探针。本研究提出了一种新的方法，即利用无标记的、基于细胞游离酵母嵌入抗体样片段（yeast-scFv）的电化学检测方法，对肠道病原体溶组织内阿米巴的一种蛋白进行检测。
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