Department of Neurosurgery, Heinrich-Heine-University, Düsseldorf, Germany.
Stroke. 2013 Mar;44(3):799-802. doi: 10.1161/STROKEAHA.112.673806. Epub 2013 Jan 17.
There is a controversy about the time span over which cerebral aneurysms develop. In particular, it is unknown whether collagen in ruptured aneurysms undergoes more rapid turnover than in unruptured aneurysms.(14)C birth dating of collagen could be used to address this question.
Aneurysmal domes from patients undergoing surgical treatment for ruptured or unruptured aneurysms were excised. Aneurysmal collagen was isolated and purified after pepsin digestion. Collagen from mouse tendons served as controls. F(14)C levels in collagen were analyzed by accelerator mass spectrometry and correlated with patient age and aneurysm size.
Analysis of 10 aneurysms from 9 patients (6 ruptured, 3 unruptured) revealed an average aneurysm collagen age of <5 years, generally irrespective of patient age and aneurysm size or rupture status. Interestingly, F(14)C levels correlated with patient age as well as aneurysm size in ruptured aneurysm collagen samples.
Our preliminary data suggest that collagen extracted from intracranial aneurysms generally has a high turnover, associated with aneurysm size and patient age. The correlation of patient age and aneurysm F(14)C levels could explain models of aneurysm development. Although preliminary, our findings may have implications for the biological and structural stability of ruptured and unruptured intracranial aneurysms.
关于颅内动脉瘤的发展时间跨度存在争议。特别是，破裂动脉瘤中的胶原蛋白是否比未破裂动脉瘤中的胶原蛋白具有更快的周转率尚不清楚。(14)C 胶原的出生日期可以用来解决这个问题。
从接受手术治疗破裂或未破裂动脉瘤的患者中切除动脉瘤穹顶。胃蛋白酶消化后，从动脉瘤中分离和纯化胶原蛋白。鼠肌腱中的胶原蛋白作为对照。通过加速器质谱分析胶原蛋白中的 F(14)C 水平，并与患者年龄和动脉瘤大小相关。
对 9 名患者的 10 个动脉瘤（6 个破裂，3 个未破裂）进行分析，发现平均动脉瘤胶原年龄<5 年，通常与患者年龄以及动脉瘤大小或破裂状态无关。有趣的是，F(14)C 水平与破裂动脉瘤胶原样本中的患者年龄和动脉瘤大小相关。
我们的初步数据表明，从颅内动脉瘤中提取的胶原蛋白通常具有较高的周转率，与动脉瘤大小和患者年龄有关。患者年龄和动脉瘤 F(14)C 水平的相关性可以解释动脉瘤发展的模型。尽管初步，但我们的发现可能对破裂和未破裂颅内动脉瘤的生物学和结构稳定性具有影响。
