Institute of Oral Biology, Kyung Hee University, Seoul, Republic of Korea.
Department of Maxillofacial Biomedical Engineering, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
J Med Microbiol. 2013 Sep;62(Pt 9):1307-1316. doi: 10.1099/jmm.0.053553-0. Epub 2013 Jan 17.
Prevotella intermedia, a major periodontopathogen, has been shown to be resistant to many antibiotics. In the present study, we examined the effect of the FDA-approved iron chelators deferoxamine (DFO) and deferasirox (DFRA) against planktonic and biofilm cells of P. intermedia in order to evaluate the possibility of using these iron chelators as alternative control agents against P. intermedia. DFRA showed strong antimicrobial activity (MIC and MBC values of 0.16 mg ml(-1)) against planktonic P. intermedia. At subMICs, DFRA partially inhibited the bacterial growth and considerably prolonged the bacterial doubling time. DFO was unable to completely inhibit the bacterial growth in the concentration range tested and was not bactericidal. Crystal violet binding assay for the assessment of biofilm formation by P. intermedia showed that DFRA significantly decreased the biofilm-forming activity as well as the biofilm formation, while DFO was less effective. DFRA was chosen for further study. In the ATP-bioluminescent assay, which reflects viable cell counts, subMICs of DFRA significantly decreased the bioactivity of biofilms in a concentration-dependent manner. Under the scanning electron microscope, P. intermedia cells in DFRA-treated biofilm were significantly elongated compared to those in untreated biofilm. Further experiments are necessary to show that iron chelators may be used as a therapeutic agent for periodontal disease.
中间普氏菌是一种主要的牙周病原菌，已被证明对许多抗生素具有耐药性。在本研究中，我们研究了 FDA 批准的铁螯合剂去铁胺（DFO）和地拉罗司（DFRA）对中间普氏菌浮游和生物膜细胞的影响，以评估这些铁螯合剂作为中间普氏菌替代控制剂的可能性。DFRA 对浮游中间普氏菌表现出很强的抗菌活性（MIC 和 MBC 值为 0.16 mg ml(-1))。在亚 MIC 浓度下，DFRA 部分抑制细菌生长并显著延长细菌倍增时间。DFO 不能在测试浓度范围内完全抑制细菌生长，也没有杀菌作用。用于评估中间普氏菌生物膜形成的结晶紫结合测定表明，DFRA 显著降低了生物膜形成活性和生物膜形成，而 DFO 的效果较差。选择 DFRA 进行进一步研究。在反映活细胞计数的 ATP 生物发光测定中，DFRA 的亚 MIC 浓度依赖性地显著降低了生物膜的生物活性。在扫描电子显微镜下，与未处理的生物膜相比，DFRA 处理的生物膜中的中间普氏菌细胞明显伸长。需要进一步的实验来表明铁螯合剂可用作牙周病的治疗剂。
