Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland.
J Med Microbiol. 2013 May;62(Pt 5):773-777. doi: 10.1099/jmm.0.049361-0. Epub 2013 Jan 17.
An ammonium chloride procedure was used to prepare a bacterial pellet from positive blood cultures, which was used for direct inoculation of VITEK 2 cards. Correct identification reached 99% for Enterobacteriaceae and 74% for staphylococci. For antibiotic susceptibility testing, very major and major errors were 0.1 and 0.3% for Enterobacteriaceae, and 0.7 and 0.1% for staphylococci, respectively. Thus, bacterial pellets prepared with ammonium chloride allow direct inoculation of VITEK cards with excellent accuracy for Enterobacteriaceae and a lower accuracy for staphylococci.
采用氯化铵法从阳性血培养物中制备细菌沉淀，用于直接接种 VITEK 2 卡。肠杆菌科的正确鉴定率达到 99%，葡萄球菌达到 74%。对于抗生素敏感性测试，肠杆菌科的非常大错误和主要错误分别为 0.1%和 0.3%，葡萄球菌分别为 0.7%和 0.1%。因此，用氯化铵制备的细菌沉淀可直接接种 VITEK 卡，肠杆菌科的准确性非常高，葡萄球菌的准确性较低。
