Shirtcliffe N J, Roach P
Biomimetic Materials, Hochschule Rhein-Waal, Rhine-Waal University of Applied Sciences, Kleve, Germany.
Methods Mol Biol. 2013;949:269-81. doi: 10.1007/978-1-62703-134-9_18.
Fouling of surfaces is often problematic in microfluidic devices, particularly when using protein or -enzymatic solutions. Various coating methods have been investigated to reduce the tendency for protein molecules to adsorb, mostly relying on hydrophobic surface chemistry or the antifouling ability of -polyethylene glycol. Here we present the potential use of superhydrophobic surfaces to not only reduce the amount of surface contamination but also to induce self-cleaning under flow conditions. The methodology is presented in order to prepare superhydrophobic surface coatings having micro- and nanoscale feature dimensions, as well as a step-by-step guide to quantify adsorbed protein down to nanogram levels. The fabrication of these surfaces as coatings via silica sol-gel and copper nano-hair growth is presented, which can be applied within microfluidic devices manufactured from various materials.
在微流控设备中，表面污染问题常常很棘手，尤其是在使用蛋白质或酶溶液时。人们已经研究了各种涂层方法来降低蛋白质分子吸附的倾向，主要依靠疏水表面化学或聚乙二醇的抗污染能力。在此，我们展示了超疏水表面的潜在用途，它不仅可以减少表面污染量，还能在流动条件下实现自清洁。本文介绍了制备具有微米和纳米级特征尺寸的超疏水表面涂层的方法，以及将吸附蛋白质定量至纳克水平的分步指南。还介绍了通过硅溶胶 - 凝胶和铜纳米毛发状生长将这些表面制成涂层的方法，该方法可应用于由各种材料制造的微流控设备中。
