Department of Medicine, Stem Cell Facility, State University of New York at Stony Brook , Stony Brook, NY , USA.
Nanotoxicology. 2014 Mar;8(2):189-201. doi: 10.3109/17435390.2013.769128. Epub 2013 Feb 8.
Gold nanoparticles (AuNPs) are currently used in numerous medical applications. Herein, we describe their in vitro impact on human adipose-derived stromal cells (ADSCs) using 13 nm and 45 nm citrate-coated AuNPs. In their non-differentiated state, ADSCs were penetrated by the AuNPs and stored in vacuoles. The presence of the AuNPs in ADSCs resulted in increased population doubling times, decreased cell motility and cell-mediated collagen contraction. The degree to which the cells were impacted was a function of particle concentration, where the smaller particles required a sevenfold higher concentration to have the same effect as the larger ones. Furthermore, AuNPs reduced adipogenesis as measured by lipid droplet accumulation and adiponectin secretion. These effects correlated with transient increases in DLK1 and with relative reductions in fibronectin. Upon removal of exogenous AuNPs, cellular NP levels decreased and normal ADSC functions were restored. As adiponectin helps regulate energy metabolism, local fluctuations triggered by AuNPs can lead to systemic changes. Hence, careful choice of size, concentration and clinical application duration of AuNPs is warranted.
金纳米粒子（AuNPs）目前在许多医学应用中得到使用。在此，我们使用 13nm 和 45nm 柠檬酸包覆的 AuNPs 描述了它们对人脂肪来源基质细胞（ADSCs）的体外影响。在未分化状态下，AuNPs 穿透 ADSCs 并储存在空泡中。AuNPs 在 ADSCs 中的存在导致倍增时间增加、细胞迁移能力降低和细胞介导的胶原收缩减少。细胞受到影响的程度是粒子浓度的函数，其中较小的粒子需要高出七倍的浓度才能产生与较大粒子相同的效果。此外，AuNPs 减少了脂滴积累和脂联素分泌所测量的脂肪生成。这些作用与 DLK1 的短暂增加和纤连蛋白的相对减少有关。去除外源性 AuNPs 后，细胞内 NP 水平降低，ADSCs 恢复正常功能。由于脂联素有助于调节能量代谢，AuNPs 引发的局部波动可能导致全身变化。因此，有必要仔细选择 AuNPs 的尺寸、浓度和临床应用持续时间。
