MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China.
Langmuir. 2013 Jul 23;29(29):9138-48. doi: 10.1021/la401556k. Epub 2013 Jul 11.
With the development of nanotechnology and its application in biomedicine, studies on the interaction between nanoparticles and cells have become increasingly important. To understand the surface and size effects on cell interaction of nanoparticles, the cellular uptake behaviors of two series of gold nanoparticles (AuNPs) with both positively and negatively charged surfaces and sizes range from ~16 to ~58 nm were investigated in both phagocytic RAW 264.7 and nonphagocytic HepG2 cells. The internalization of AuNPs was quantified by ICP-MS, and the intracellular fate of NPs was evaluated by TEM analysis. The results showed that the AuNPs with positive surface charge have much higher cell internalization ability than those with negative surface charge in nonphagocytic HepG2 cells. However, the uptake extent of negatively charged AuNPs was similar with that of the positively charged AuNPs when in phagocytic RAW 264.7 cells. Among the tested size range, negatively charged AuNPs with a diameter of ~40 nm had the highest uptake in both cells, while the positively charged AuNPs did not show a certain tendency. Intracellular TEM analysis demonstrated the different fate of AuNPs in different cells, where both the positively and negatively charged AuNPs were mainly trapped in the lysosomes in HepG2 cells, but many of them were localized in phagosomes when in RAW 264.7 cells. Cytotoxicity of these AuNPs was tested by both MTT and LDH assays, which suggested NP's toxicity is closely related to the tested cell types besides the surface and size of NPs. It demonstrates that cell interaction between nanoparticles and cells is not only affected by surface and size factors but also strongly depends on cell types.
随着纳米技术的发展及其在生物医学中的应用，研究纳米粒子与细胞的相互作用变得越来越重要。为了了解纳米粒子表面和尺寸对细胞相互作用的影响，研究了具有正、负表面电荷和尺寸范围为16 至58nm 的两系列金纳米粒子（AuNPs）在吞噬 RAW 264.7 和非吞噬 HepG2 细胞中的摄取行为。通过 ICP-MS 定量测定 AuNPs 的内化，通过 TEM 分析评估 NPs 的细胞内命运。结果表明，在非吞噬性 HepG2 细胞中，具有正表面电荷的 AuNPs 的细胞内化能力远高于具有负表面电荷的 AuNPs。然而，在吞噬性 RAW 264.7 细胞中，带负电荷的 AuNPs 的摄取程度与带正电荷的 AuNPs 相似。在测试的尺寸范围内，带负电荷的直径约为 40nm 的 AuNPs 在两种细胞中的摄取量最高，而带正电荷的 AuNPs 则没有表现出一定的趋势。细胞内 TEM 分析表明，不同细胞中 AuNPs 的命运不同，带正、负电荷的 AuNPs 主要在 HepG2 细胞的溶酶体中被捕获，但在 RAW 264.7 细胞中，许多 AuNPs 被定位在吞噬体中。通过 MTT 和 LDH 测定法测试了这些 AuNPs 的细胞毒性，这表明 NP 的毒性不仅与 NP 的表面和尺寸有关，还与所测试的细胞类型密切相关。这表明纳米粒子与细胞之间的细胞相互作用不仅受表面和尺寸因素的影响，还强烈依赖于细胞类型。
