Department of Radiotherapy/Oncology, Radiobiology and Radiopathology Unit, Democritus University of Thrace, Alexandroupolis, Greece.
Biochem Biophys Res Commun. 2013 Feb 15;431(3):456-9. doi: 10.1016/j.bbrc.2012.12.146. Epub 2013 Jan 16.
Microplates are useful tools in chemistry, biotechnology and molecular biology. In radiobiology research, these can be also applied to assess the effect of a certain radiation dose delivered to the whole microplate, to test radio-sensitivity, radio-sensitization or radio-protection. Whether different radiation doses can be accurately applied to a single 96-well plate to further facilitate and accelerated research by one hand and spare funds on the other, is a question dealt in the current paper. Following repeated ion-chamber, TLD and radiotherapy planning dosimetry we established a method for multi-dose irradiation of cell cultures within a 96-well plate, which allows an accurate delivery of desired doses in sequential columns of the microplate. Up to eight different dose levels can be tested in one microplate. This method results in fast and reliable estimation of radiation dose-response curves.
微孔板在化学、生物技术和分子生物学领域是非常有用的工具。在放射生物学研究中，微孔板也可用于评估整个微孔板所接受的特定辐射剂量的影响，以测试放射敏感性、放射增敏或放射保护。能否将不同的辐射剂量准确地应用于单个 96 孔板，一方面可以进一步促进和加速研究，另一方面可以节省资金，这是当前研究中要解决的问题。通过重复电离室、TLD 和放射治疗计划剂量学，我们建立了一种在 96 孔板内进行细胞培养多剂量照射的方法，该方法可以在微孔板的连续列中准确地输送所需剂量。一个微孔板中最多可以测试八个不同的剂量水平。这种方法可以快速可靠地估计辐射剂量-反应曲线。
