使用活力测定法评估肺癌和成纤维细胞系中的放射生物学α/β比值
Assessment of Radiobiological α/β Ratio in Lung Cancer and Fibroblast Cell Lines Using Viability Assays.
作者信息
Karagounis Ilias V, Skourti Eleni K, Liousia Maria V, Koukourakis Michael I
机构信息
Department of Radiotherapy/Oncology, Democritus University of Thrace, Alexandroupolis, Greece.
Department of Radiotherapy/Oncology, Democritus University of Thrace, Alexandroupolis, Greece
出版信息
In Vivo. 2017 Mar-Apr;31(2):175-179. doi: 10.21873/invivo.11042.
BACKGROUND/AIM: Altered fractionation is an area of intense clinical research in radiation oncology. Estimation of the α/β ratio of individual carcinomas after establishment of primary cell cultures from tumor biopsies may prove of importance in the individualization of radiotherapy schemes.
MATERIALS AND METHODS
Here we proposed a simple method to estimate the α/β ratio in cultured cell lines (two lung carcinomas: A549 and H1299; one lung fibroblast cell line: MRC5), using viability assays.
RESULTS
For the A549 cell line, the α/β ratio ranged from 14-25 Gy, for H1299 from 11-43 Gy and for the MRC5 fibroblast cell line this was far lower, ranging from 0.69 to 6 Gy. The α/β ratio decreased when extracted from comparisons of lower dose per fraction schemes.
CONCLUSION
The α/β ratio of a cell line can be easily defined after simple viability/dose fractionation experiments.
背景/目的：分割方式的改变是放射肿瘤学中临床研究的一个热点领域。从肿瘤活检标本建立原代细胞培养后，估计个体癌组织的α/β比值可能对放疗方案的个体化具有重要意义。
材料与方法
在此，我们提出了一种使用活力测定法来估计培养细胞系（两种肺癌细胞系：A549和H1299；一种肺成纤维细胞系：MRC5）中α/β比值的简单方法。
结果
对于A549细胞系，α/β比值范围为14 - 25 Gy，H1299为11 - 43 Gy，而MRC5成纤维细胞系的该比值则低得多，范围为0.69至6 Gy。当从较低分次剂量方案的比较中提取时，α/β比值降低。
结论
经过简单的活力/剂量分割实验后，细胞系的α/β比值可以很容易地确定。
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