Université de Toulouse, Laboratoire des Interactions Moléculaires et Réactivité Chimique et Photochimique, UMR CNRS 5623, Université Paul-Sabatier, 118 route de Narbonne, F-31062 Toulouse, France.
Food Chem Toxicol. 2013 May;55:202-8. doi: 10.1016/j.fct.2013.01.004. Epub 2013 Jan 16.
In this study, biological activities of methanolic extracts from Artemisia herba-alba, Ruta chalpensis L. and Peganum harmala L. plants, collected in Centre of Tunisia, were investigated. Results showed an important phenolic composition of Artemisia herba-alba (123.95±4.3g GAE/kg of dry mass). The extract of this plant showed, using different antioxidant assays (DPPH, ABTS and AAPH/linoleic acid methods) and an IFN-γ/LPS induced RAW 264.7 murine macrophages' assay, the highest antioxidant (IC50 (DPPH assay) 20.64±0.84mg/L) and anti-inflammatory (72% inhibition at 150mg/L) activities, respectively. Excepting Peganum harmala L. extract, the two other extracts showed a high anticancer activity against several cell lines (human bladder carcinoma RT112, human laryngeal carcinoma Hep2 and human myelogenous leukemia K562), for A. herba-laba IC50=81.59±4.4, 59.05±3.66 and 90.96mg/L respectively, but not on normal peripheral blood mononuclear cells. All these biological activities are well correlated with the phenolic contents of these extracts. These findings demonstrate the remarkable potential of these plants as valuable source of antioxidants with exhibit original and interesting anti-inflammatory and anticancer capacities.
在这项研究中，研究了产自突尼斯中心的艾草（Artemisia herba-alba）、山金车（Ruta chalpensis L.）和骆驼蓬（Peganum harmala L.）植物的甲醇提取物的生物活性。结果表明，艾草提取物含有丰富的酚类化合物（123.95±4.3g GAE/kg 干重）。该植物提取物在使用不同抗氧化测定法（DPPH、ABTS 和 AAPH/亚油酸法）和 IFN-γ/LPS 诱导的 RAW 264.7 鼠巨噬细胞测定法进行测试时，表现出最强的抗氧化（IC50（DPPH 测定法）为 20.64±0.84mg/L）和抗炎（在 150mg/L 时抑制率为 72%）活性。除了骆驼蓬提取物外，其他两种提取物对几种细胞系（人膀胱癌 RT112、人喉癌细胞 Hep2 和人髓性白血病 K562）均显示出较高的抗癌活性，艾草提取物的 IC50 值分别为 81.59±4.4、59.05±3.66 和 90.96mg/L，但对正常外周血单核细胞没有活性。这些生物活性与这些提取物的酚类含量密切相关。这些发现表明，这些植物具有作为抗氧化剂的巨大潜力，具有独特而有趣的抗炎和抗癌能力。
