Bou Malhab Lara J, Harb Amani A, Eldohaji Leen, Taneera Jalal, Al-Hroub Hamza M, Abuhelwa Ahmad, Alzoubi Karem H, Abu-Irmaileh Bashaer, Hudaib Mohammad, Almaliti Jehad, Abdel-Rahman Wael M, Shanableh Abdallah, Semreen Mohammad H, El-Huneidi Waseem, Abu-Gharbieh Eman, Bustanji Yasser
Sharjah Institute for Medical Research University of Sharjah Sharjah UAE.
Department of Basic Sciences, Faculty of Arts and Sciences Al-Ahliyya Amman University Amman Jordan.
Food Sci Nutr. 2024 Dec 31;13(1):e4715. doi: 10.1002/fsn3.4715. eCollection 2025 Jan.
Colorectal cancer (CRC) is a prevalent and deadly disease, necessitating the exploration of novel therapeutic strategies. Traditional chemotherapy often encounters drug resistance and adverse side effects, highlighting the need for alternative approaches. , a plant rich in phytochemical constituents, was investigated for its potential as an anticancer agent against colorectal cancer (CRC). The primary objective of this study was to investigate the cytotoxic effects of the methanolic extract of on eight CRC cell lines including: Caco-2, DLD1, RKOp53, RKOp53, HCTp53, HCTp53, SW620, and SW480. Specifically, the study investigated the extract's impact on cell viability, apoptosis, cell cycle progression, and effects on the PI3K/AKT/mTOR signaling pathway. Chemical derivatization and Gas Chromatography-Mass Spectrometry (GC-MS) analysis revealed a diverse array of bioactive compounds, including ephedrine, hydroxyflavone, quinolinic acid, 4-hydroxybenzoic acid, borneol, β-eudesmol, and camphor, known for their cytotoxic properties. The methanolic extract of exhibited varying degrees of cytotoxicity across a panel of CRC cell lines, with IC values indicating differential sensitivity. The extract triggered apoptosis in many cell lines, irrespective of p53 status. Importantly, extract caused G2-M phase cell cycle arrest in CRC cells, accompanied by a decrease in Cyclin B1 and CDK1 expression. Furthermore, the extract demonstrated an inhibitory effect on the PI3K/AKT/mTOR pathway, crucial in cancer progression. These findings highlight the promising anticancer potential of as a valuable resource for innovative CRC treatments. Further research is warranted to elucidate its specific anticancer characteristics and explore its potential incorporation into future cancer therapy approaches.
结直肠癌（CRC）是一种常见且致命的疾病，因此需要探索新的治疗策略。传统化疗常常会遇到耐药性和不良副作用，这凸显了采用替代方法的必要性。对一种富含植物化学成分的植物进行了研究，以探讨其作为抗结直肠癌（CRC）抗癌剂的潜力。本研究的主要目的是研究该植物甲醇提取物对八种CRC细胞系的细胞毒性作用，这些细胞系包括：Caco-2、DLD1、RKOp53、RKOp53、HCTp53、HCTp53、SW620和SW480。具体而言，该研究调查了提取物对细胞活力、凋亡、细胞周期进程的影响以及对PI3K/AKT/mTOR信号通路的作用。化学衍生化和气相色谱-质谱（GC-MS）分析揭示了一系列生物活性化合物，包括麻黄碱、羟基黄酮、喹啉酸、4-羟基苯甲酸、冰片、β-桉叶醇和樟脑，它们以其细胞毒性特性而闻名。该植物的甲醇提取物在一组CRC细胞系中表现出不同程度的细胞毒性，IC值表明了不同的敏感性。该提取物在许多细胞系中引发凋亡，与p53状态无关。重要的是，该植物提取物导致CRC细胞在G2-M期细胞周期停滞，同时细胞周期蛋白B1和细胞周期蛋白依赖性激酶1（CDK1）表达下降。此外，该提取物对PI3K/AKT/mTOR通路具有抑制作用，该通路在癌症进展中至关重要。这些发现突出了该植物作为创新CRC治疗的宝贵资源具有有前景的抗癌潜力。有必要进行进一步研究以阐明其具体的抗癌特性，并探索将其纳入未来癌症治疗方法的可能性。
