Forest Products Biotechnology/Bioenergy Group, Faculty of Forestry, University of British Columbia, 2424 Main Mall, Vancouver BC, Canada V6T 1Z4.
Bioresour Technol. 2013 Feb;130:570-7. doi: 10.1016/j.biortech.2012.12.093. Epub 2012 Dec 21.
A two-stage pretreatment approach, employing steam followed by organosolv treatment, was assessed for its ability to fractionate and recover most of the hemicellulose, lignin and cellulose components of poplar wood chips. A mild steaming stage was initially used to maximise hemicellulose sugar recovery, with 63% of the original xylan solubilised and recovered after this stage and close to 90% recovered in total. Rather than hindering subsequent organosolv delignification, the prior steam treatment enhanced lignin solubilisation with more than 66% of the original lignin removed after the two-stage pretreatment. The extracted lignin contained at least equal or greater amounts of functional groups as compared to the lignin solubilised after a single-stage organosolv pretreatment. More than 98% of the original cellulose was recovered after the two-stage pretreatment and 88% of the cellulose could be hydrolysed to glucose at enzyme loading of 5FPU/g cellulose after 72h.
两段式预处理方法，采用蒸汽预处理后接着进行有机溶剂预处理，用于分离和回收杨木木屑中大部分半纤维素、木质素和纤维素成分。首先采用温和的蒸汽预处理阶段，使半纤维素糖的回收率最大化，在此阶段后有 63%的原木聚糖溶解并回收，总共回收接近 90%。与阻碍后续有机溶剂脱木质素不同，先前的蒸汽预处理增强了木质素的溶解，在两段式预处理后去除了超过 66%的原木质素。提取的木质素与单一阶段有机溶剂预处理后溶解的木质素相比，至少含有同等或更多数量的官能团。两段式预处理后，超过 98%的原纤维素得到回收，在酶用量为 5FPU/g 纤维素，72 小时后，88%的纤维素可以水解为葡萄糖。
