鹰嘴豆芽异黄酮提取物对去卵巢大鼠雌激素活性的评估。
Assessment of the estrogenic activities of chickpea (Cicer arietinum L) sprout isoflavone extract in ovariectomized rats.
机构信息
Xinjiang Key Laboratory of Plant Resources and Natural Products Chemistry, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, Urumqi 830011, China.
出版信息
Acta Pharmacol Sin. 2013 Mar;34(3):380-6. doi: 10.1038/aps.2012.160. Epub 2013 Jan 21.
AIM
Chickpea (Cicer arietinum L) is a traditional Uighur herb. In this study we investigated the estrogenic activities of the isoflavones extracted from chickpea sprouts (ICS) in ovariectomized rats.
METHODS
Ten-week-old virgin Sprague-Dawley female rats were ovariectomized (OVX). The rats were administered via intragastric gavage 3 different doses of ICS (20, 50, or 100 mg·kg(-1)·d(-1)) for 5 weeks. Their uterine weight and serum levels of 17β-estradiol (E2), follicle stimulating hormone (FSH) and luteinizing hormone (LH) were measured. The epithelial height, number of glands in the uterus, and number of osteoclasts in the femur were histologically quantified, and the expression of proliferating cell nuclear antigen (PCNA) was assessed immunohistochemically. Bone structural parameters, including bone mineral density (BMD), bone volume/tissue volume (BV/TV), trabecular thickness (Tb.Th) and trabecular separation (Tb.Sp) were measured using Micro-CT scanning.
RESULTS
Treatments of OVX rats with ICS (50 or 100 mg·kg(-1)·d(-1)) produced significant estrogenic effects on the uteruses, including the increases in uterine weight, epithelial height and gland number, as well as in the expression of the cell proliferation marker PCNA. The treatments changed the secretory profile of ovarian hormones and pituitary gonadotropins: serum E2 level was significantly increased, while serum LH and FSH levels were decreased compared with the vehicle-treated OVX rats. Furthermore, the treatments significantly attenuated the bone loss, increased BMD, BV/TV and Tb.Th and decreased Tb.Sp and the number of osteoclasts. Treatment of OVX rats with the positive control drug E2 (0.25 mg·kg(-1)·d(-1)) produced similar, but more prominent effects.
CONCLUSION
ICS exhibits moderate estrogenic activities as compared to E2 in ovariectomized rats, suggesting the potential use of ICS for the treatment of menopausal symptoms and osteoporosis caused by estrogen deficiency.
目的
鹰嘴豆（Cicer arietinum L）是一种传统的维吾尔草药。本研究旨在探讨从鹰嘴豆芽中提取的异黄酮（ICS）在去卵巢大鼠中的雌激素活性。
方法
10 周龄的处女 Sprague-Dawley 雌性大鼠行卵巢切除术（OVX）。大鼠通过灌胃给予 3 种不同剂量的 ICS（20、50 或 100mg·kg(-1)·d(-1)），持续 5 周。测量其子宫重量和血清 17β-雌二醇（E2）、卵泡刺激素（FSH）和黄体生成素（LH）水平。组织学定量测量子宫上皮高度、腺体数量和股骨破骨细胞数量，并免疫组织化学评估增殖细胞核抗原（PCNA）的表达。使用 Micro-CT 扫描测量骨结构参数，包括骨密度（BMD）、骨体积/组织体积（BV/TV）、骨小梁厚度（Tb.Th）和骨小梁间距（Tb.Sp）。
结果
ICS（50 或 100mg·kg(-1)·d(-1)）治疗 OVX 大鼠对子宫产生显著的雌激素作用，包括子宫重量、上皮高度和腺体数量的增加，以及细胞增殖标志物 PCNA 的表达增加。这些治疗改变了卵巢激素和垂体促性腺激素的分泌谱：血清 E2 水平显著升高，而血清 LH 和 FSH 水平与 vehicle 处理的 OVX 大鼠相比降低。此外，这些治疗显著减轻了骨丢失，增加了 BMD、BV/TV 和 Tb.Th，降低了 Tb.Sp 和破骨细胞数量。阳性对照药物 E2（0.25mg·kg(-1)·d(-1)）治疗 OVX 大鼠产生了类似但更显著的作用。
结论
与 E2 相比，ICS 在去卵巢大鼠中表现出中等强度的雌激素活性，提示 ICS 可能用于治疗因雌激素缺乏引起的绝经症状和骨质疏松症。
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