Ballesteros-Gómez Ana, Rubio Soledad, Pérez-Bendito Dolores
Department of Analytical Chemistry, Facultad de Ciencias, Edificio Anexo Marie Curie, Campus de Rabanales, 14171 Córdoba, Spain.
J Chromatogr A. 2009 Jan 16;1216(3):449-69. doi: 10.1016/j.chroma.2008.06.037. Epub 2008 Jul 3.
Food constitutes the primary route for human exposure to bisphenol A (BPA), one of the highest volume chemicals produced worldwide. The estrogenic properties of BPA, its wide dispersive use and the recent extensive literature describing low-dose BPA effects in animals, have raised concerns about its possible adverse effects on human health. A reliable health risk assessment of BPA relies basically on its unambiguous identification and accurate quantification in food, and the aim of the present review is to give an overview of the analytical methods reported so far for the determination of BPA in these matrices. Emphasis is placed on the main strategies developed for sample treatment, which usually consists of several laborious and time-consuming steps in order to achieve the required sensitivity and selectivity. Separation, identification and quantitation of BPA is today reliably made with mass spectrometric methods, namely liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS), and thus main attention is devoted to these techniques, but other methods using LC coupled to fluorescence or electrochemical detection, as well as immunochemical methods are also covered. Recent and expected future developments are discussed.
食物是人类接触双酚A（BPA）的主要途径，双酚A是全球产量最高的化学品之一。双酚A的雌激素特性、其广泛的分散使用以及近期大量描述双酚A对动物低剂量影响的文献，引发了人们对其可能对人类健康产生的不利影响的担忧。对双酚A进行可靠的健康风险评估基本上依赖于在食物中对其进行明确鉴定和准确定量，本综述的目的是概述迄今为止报道的用于测定这些基质中双酚A的分析方法。重点是为样品处理开发的主要策略，为了达到所需的灵敏度和选择性，样品处理通常包括几个繁琐且耗时的步骤。如今，双酚A的分离、鉴定和定量可通过质谱方法可靠地完成，即液相色谱 - 质谱联用（LC - MS）和气相色谱 - 质谱联用（GC - MS），因此主要关注这些技术，但也涵盖了使用液相色谱与荧光或电化学检测联用的其他方法以及免疫化学方法。还讨论了近期以及预期未来的发展情况。
