Defluviimonas aestuarii sp. nov., a marine bacterium isolated from a tidal flat, and emended description of the genus Defluviimonas Foesel et al. 2011.

A novel Gram-staining-negative, strictly aerobic bacterium, designated BS14(T), was isolated from a marine tidal flat of the South Sea in Korea. Colonies were opaque, white, smooth and circular on marine agar. Cells were moderately halophilic, non-motile rods showing catalase- and oxidase-positive reactions. Growth of strain BS14(T) was observed at 5-40 °C (optimum: 30 °C), pH 6.5-9.5 (optimum: 7.0-7.5) and 0-10 % (w/v) NaCl (optimum: 1-1.5 %). The G+C content of the genomic DNA was 61.6 mol%. Strain BS14(T) contained ubiquinone-10 (Q-10) as the sole respiratory quinone and summed feature 8 (comprising C18 : 1ω7c and/or C18 : 1ω6c), C18 : 0 3-OH, C10 : 0 3-OH and C18 : 0 as the major fatty acids. The polar lipid pattern comprised phosphatidylethanolamine, diphosphatidylglycerol, an unidentified aminolipid, an unidentified phospholipid and an unidentified polar lipid. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BS14(T) formed a tight phylogenetic lineage with Defluviimonas denitrificans D9-3(T) with a bootstrap value of 100 %. The 16S rRNA gene sequence similarity between strain BS14(T) and D. denitrificans D9-3(T) was 97.4 % and their DNA-DNA relatedness was 19.1 ± 3.6 %. Based on the phenotypic and genotypic studies, strain BS14(T) represents a novel species of the genus Defluviimonas, for which the name Defluviimonas aestuarii sp. nov. is proposed. The type strain is BS14(T) (= KACC 16442(T) = JCM 18630(T)). An emended description of the genus Defluviimonas Foesel et al. 2011 is also proposed.