School of Biological Sciences and Research Center for Biomolecules and Biosystems, Chung-Ang University, Seoul 156-756, Republic of Korea.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4012-4017. doi: 10.1099/ijs.0.048561-0. Epub 2013 May 24.
A Gram-negative, strictly aerobic, motile bacterium with flagella, designated strain G210(T), was isolated from a crude-oil-contaminated tidal flat of the Taean coast in South Korea. Cells were non-spore-forming, ovoid rods showing catalase- and oxidase-positive reactions. Growth of strain G210(T) was observed between 15 and 45 °C (optimum, 30-35 °C) and between pH 5.5 and 9.0 (optimum, pH 6.5-7.5). Strain G210(T) contained Q-10 as the sole isoprenoid quinone and summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c) and C19 : 0 cyclo ω8c as the major fatty acids. Putrescine and spermidine were identified as the major polyamines. Strain G210(T) contained phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, an unidentified phospholipid and an unidentified aminolipid as polar lipids. The G+C content of the genomic DNA was 56.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain G210(T) formed a phyletic lineage with members of the genus Aquamicrobium. Strain G210(T) was most closely related to Aquamicrobium ahrensii 905/1(T) (97.9 % similarity). The DNA-DNA relatedness between strain G210(T) and the type strain of A. ahrensii was 24±0.5 %. On the basis of phenotypic, chemotaxonomic and molecular properties, strain G210(T) represents a novel species within the genus Aquamicrobium, for which the name Aquamicrobium aestuarii sp. nov. is proposed. The type strain is G210(T) ( = KACC 14931(T) = JCM 16876(T)).
一株革兰氏阴性、严格需氧、运动的细菌，带有鞭毛，被命名为 G210(T) 株，从韩国泰安海岸的一个原油污染的潮滩中分离得到。细胞是非孢子形成的卵形杆状，呈过氧化氢酶和氧化酶阳性反应。G210(T) 株的生长温度为 15 到 45°C（最佳温度为 30-35°C），pH 值为 5.5 到 9.0（最佳 pH 值为 6.5-7.5）。G210(T) 株含有 Q-10 作为唯一的异戊二烯醌，并含有 summed feature 8（C18：1ω7c 和/或 C18：1ω6c）和 C19：0 环 ω8c 作为主要脂肪酸。腐胺和亚精胺被鉴定为主要的多胺。G210(T) 株含有磷脂酰甘油、双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、磷脂单甲醚乙醇胺、一种未鉴定的磷脂和一种未鉴定的氨基脂作为极性脂。基因组 DNA 的 G+C 含量为 56.9 mol%。基于 16S rRNA 基因序列的系统发育分析表明，G210(T) 株与 Aquamicrobium 属的成员形成一个分支。G210(T) 株与 Aquamicrobium ahrensii 905/1(T)（97.9%相似度）最为密切相关。G210(T) 株与 A. ahrensii 模式株之间的 DNA-DNA 相关性为 24±0.5%。根据表型、化学分类学和分子特性，G210(T) 株代表了 Aquamicrobium 属中的一个新种，因此建议将其命名为 Aquamicrobium aestuarii sp. nov.。模式株为 G210(T)（=KACC 14931(T)=JCM 16876(T)）。
