Health care and Energy Materials Laboratory, Nanoscience and Nanotechnology Initiative, National University of Singapore, Faculty of Engineering, 2 Engineering Drive 3, Singapore 117576.
Macromol Biosci. 2013 Mar;13(3):366-75. doi: 10.1002/mabi.201200391. Epub 2013 Jan 18.
A functional scaffold fabricated is developed from natural polymers, favoring regeneration of the ischemic myocardium. Hemoglobin/gelatin/fibrinogen (Hb/gel/fib) nanofibers are fabricated by electrospinning and are characterized for morphology, scaffold composition, functional groups and hydrophilicity. It is hypothesized that ex vivo pretreatment of mesenchymal stem cells (MSCs) using 5-azacytidine and such a functional nanofibrous construct having a high oxygen-carrying potential could lead to enhanced cardiomyogenic differentiation of MSCs and result in superior biological and functional effects. The combination of a functional nanofibrous scaffold composed of natural polymers and crosslinked with a natural crosslinking agent, phytic acid, and stem cell biology may prove to be a novel therapeutic device for treatment of myocardial infarction.
我们研制了一种由天然聚合物制成的功能性支架，有利于缺血性心肌的再生。通过静电纺丝技术制备血红蛋白/明胶/纤维蛋白原（Hb/gel/fib）纳米纤维，并对其形态、支架组成、官能团和亲水性进行了表征。我们假设使用 5-氮杂胞苷对间充质干细胞（MSCs）进行体外预处理，以及使用具有高携氧能力的这种功能性纳米纤维结构，可能会导致 MSCs 的心肌生成分化增强，并产生更好的生物学和功能效果。由天然聚合物组成的功能性纳米纤维支架与天然交联剂植酸交联的组合，以及干细胞生物学，可能会成为治疗心肌梗死的新型治疗设备。
