Zhang Rongfa, Zhong Sheng, Zeng Lilan, Li Hongyu, Zhao Rongfang, Zhang Shufang, Duan Xinting, Huang Jingsong, Zhao Ying
School of Materials and Electromechanics, Jiangxi Science and Technology Normal University, Nanchang 330038, China.
Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.
Materials (Basel). 2021 Sep 30;14(19):5710. doi: 10.3390/ma14195710.
In this study, Ti-6Al-4V alloy samples were processed by micro-arc oxidation (MAO) in phytic acid (HPhy) electrolytes with the addition of different concentrations of EDTA-MgNa (NaMgY) and potassium hydroxide (KOH). The surface characterization and cytocompatibility of MAO-treated samples were evaluated systematically. HPhy is a necessary agent for MAO coating formation, and the addition of NaMgY and KOH into the electrolytes increases the surface roughness, micropore size and Mg contents in the coatings. The MAO coatings are primarily composed of anatase, rutile, MgO and Mg(PO). Magnesium (Mg) ions in the electrolytes enter into MAO coatings by diffusion and electromigration. The MAO coatings containing 2.97 at% Mg show excellent cell viability, adhesion, proliferation, alkaline phosphatase activity, extracellular matrix (ECM) mineralization and collagen secretion, but the cytocompatibility of the MAO coatings containing 6.82 at% Mg was the worst due to the excessively high Mg content. Our results revealed that MAO coatings with proper Mg contents improve the cytocompatibility of the Ti-6Al-4V alloys and have large potential in orthopedic applications.
在本研究中，采用微弧氧化（MAO）技术在添加了不同浓度乙二胺四乙酸镁钠（NaMgY）和氢氧化钾（KOH）的植酸（HPhy）电解液中对Ti-6Al-4V合金样品进行处理。系统评估了经微弧氧化处理的样品的表面特性和细胞相容性。植酸是微弧氧化涂层形成的必要试剂，向电解液中添加NaMgY和KOH会增加涂层的表面粗糙度、微孔尺寸和镁含量。微弧氧化涂层主要由锐钛矿、金红石、氧化镁和磷酸镁组成。电解液中的镁离子通过扩散和电迁移进入微弧氧化涂层。含2.97原子百分比镁的微弧氧化涂层表现出优异的细胞活力、黏附性、增殖能力、碱性磷酸酶活性、细胞外基质（ECM）矿化和胶原蛋白分泌，但含6.82原子百分比镁的微弧氧化涂层的细胞相容性最差，因为镁含量过高。我们的结果表明，具有适当镁含量的微弧氧化涂层可提高Ti-6Al-4V合金的细胞相容性，在骨科应用中具有巨大潜力。
