Department of Chemistry, University of Basel, Spitalstrasse 51, 4056 Basel, Switzerland.
Anal Chem. 2013 Feb 19;85(4):2333-9. doi: 10.1021/ac303328g. Epub 2013 Feb 4.
A portable capillary electrophoresis instrument featuring an automated, robust, valve-based injection system was developed. This significantly facilitates operation in the field compared to previous injection approaches. These generally required delicate manual operations which are difficult to perform outside the laboratory environment. The novel system relies on pressurized air for solution delivery and a micromembrane pump for sample aspiration. Contactless conductivity detection was employed for its versatility and low power requirement. The instrument has a compact design, with all components arranged in a briefcase with dimensions of 45 × 35 × 15 cm (w × d × h) and a weight of about 8 kg. It can operate continuously for 9 h in the battery-powered mode. Depending on the task at hand, the injection system allows easy optimization for high separation efficiency, for fast separations, or for low limits of detection. To illustrate these features, the separation of four anions within 16 s is demonstrated as well as the determination of nitrite below 1 μM. The determination of phosphate at a sewage treatment plant was carried out to demonstrate a field application.
研制了一种便携式毛细管电泳仪，其特点是具有自动化、稳健、基于阀的进样系统。与以前的进样方法相比，这使得在现场操作变得更加容易。这些方法通常需要精细的手动操作，在实验室环境之外很难进行。新系统依赖于加压空气进行溶液输送和微膜泵进行样品吸入。非接触式电导检测因其多功能性和低功耗要求而被采用。该仪器设计紧凑，所有组件都安装在一个公文包中，尺寸为 45×35×15 厘米（宽×深×高），重量约为 8 公斤。它可以在电池供电模式下连续运行 9 小时。根据手头的任务，进样系统允许轻松优化以实现高分离效率、快速分离或低检测限。为了说明这些特点，演示了在 16 秒内分离四种阴离子以及低于 1 μM 的亚硝酸盐的测定。在污水处理厂进行了磷酸盐的测定，以展示现场应用。
