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用于骨组织工程应用的胶原蛋白/脱矿骨粉支架及骨膜来源细胞的研发。

Development of collagen/demineralized bone powder scaffolds and periosteum-derived cells for bone tissue engineering application.

作者信息

Thitiset Thakoon, Damrongsakkul Siriporn, Bunaprasert Tanom, Leeanansaksiri Wilairat, Honsawek Sittisak

机构信息

Interdisciplinary Program in Biomedical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok 10330, Thailand.

出版信息

Int J Mol Sci. 2013 Jan 21;14(1):2056-71. doi: 10.3390/ijms14012056.

DOI:10.3390/ijms14012056
PMID:23337204
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3565365/
Abstract

The aim of this study was to investigate physical and biological properties of collagen (COL) and demineralized bone powder (DBP) scaffolds for bone tissue engineering. DBP was prepared and divided into three groups, based on various particle sizes: 75-125 µm, 125-250 µm, and 250-500 µm. DBP was homogeneously mixed with type I collagen and three-dimensional scaffolds were constructed, applying chemical crosslinking and lyophilization. Upon culture with human periosteum-derived cells (PD cells), osteogenic differentiation of PD cells was investigated using alkaline phosphatase (ALP) activity and calcium assay kits. The physical properties of the COL/DBP scaffolds were obviously different from COL scaffolds, irrespective of the size of DBP. In addition, PD cells cultured with COL scaffolds showed significantly higher cell adhesion and proliferation than those with COL/DBP scaffolds. In contrast, COL/DBP scaffolds exhibited greater osteoinductive potential than COL scaffolds. The PD cells with COL/DBP scaffolds possessed higher ALP activity than those with COL scaffolds. PD cells cultured with COL/DBP scaffolds with 250-500 mm particle size yielded the maximum calcium deposition. In conclusion, PD cells cultured on the scaffolds could exhibit osteoinductive potential. The composite scaffold of COL/DBP with 250-500 mm particle size could be considered a potential bone tissue engineering implant.

摘要

本研究的目的是调查用于骨组织工程的胶原蛋白（COL）和脱矿骨粉（DBP）支架的物理和生物学特性。制备DBP并根据不同粒径分为三组：75-125 µm、125-250 µm和250-500 µm。将DBP与I型胶原蛋白均匀混合，并应用化学交联和冻干法构建三维支架。在用人类骨膜来源的细胞（PD细胞）培养后，使用碱性磷酸酶（ALP）活性检测试剂盒和钙检测试剂盒研究PD细胞的成骨分化。无论DBP的大小如何，COL/DBP支架的物理特性明显不同于COL支架。此外，用COL支架培养的PD细胞比用COL/DBP支架培养的细胞表现出明显更高的细胞黏附和增殖。相反，COL/DBP支架比COL支架表现出更大的骨诱导潜力。使用COL/DBP支架培养的PD细胞比使用COL支架培养的细胞具有更高的ALP活性。用粒径为250-500 µm的COL/DBP支架培养的PD细胞产生的钙沉积量最大。总之，在支架上培养的PD细胞可表现出骨诱导潜力。粒径为250-500 µm的COL/DBP复合支架可被视为一种潜在的骨组织工程植入物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/4f6dd2d0abdb/ijms-14-02056f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/95712ed061a4/ijms-14-02056f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/d68ef8e68a77/ijms-14-02056f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/f9fd6525936b/ijms-14-02056f3a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/4d35ee332945/ijms-14-02056f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/2242e924e322/ijms-14-02056f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/b72c42fc8b24/ijms-14-02056f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/651590670d32/ijms-14-02056f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/fed2f766a1ca/ijms-14-02056f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/4f6dd2d0abdb/ijms-14-02056f9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/95712ed061a4/ijms-14-02056f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/d68ef8e68a77/ijms-14-02056f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/f9fd6525936b/ijms-14-02056f3a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/4d35ee332945/ijms-14-02056f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/2242e924e322/ijms-14-02056f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/b72c42fc8b24/ijms-14-02056f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/651590670d32/ijms-14-02056f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/fed2f766a1ca/ijms-14-02056f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5e19/3565365/4f6dd2d0abdb/ijms-14-02056f9.jpg

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本文引用的文献

1
Peptide-based Biopolymers in Biomedicine and Biotechnology.生物医学与生物技术中的肽基生物聚合物
Mater Sci Eng R Rep. 2008 Jan;62(4):125-155. doi: 10.1016/j.mser.2008.04.004.
2
Effects of demineralized bone matrix on proliferation and osteogenic differentiation of mesenchymal stem cells from human umbilical cord.脱矿骨基质对人脐带间充质干细胞增殖和成骨分化的影响
J Med Assoc Thai. 2006 Sep;89 Suppl 3:S189-95.
3
Content of bone morphogenetic protein-4 in human demineralized bone: relationship to donor age and ability to induce new bone formation.
Effect of Collagen and GelMA on Preservation of the Costal Chondrocytes' Phenotype in a Scaffold .
胶原蛋白和 GelMA 对支架中环骨细胞表型保存的影响。
Sovrem Tekhnologii Med. 2023;15(2):5-16. doi: 10.17691/stm2023.15.2.01. Epub 2023 Mar 29.
4
Analysis of the Ability of Different Allografts to Act as Carrier Grafts for Local Drug Delivery.不同同种异体移植物作为局部给药载体移植物的能力分析。
J Funct Biomater. 2023 Jun 1;14(6):305. doi: 10.3390/jfb14060305.
5
Decellularized Extracellular Matrix for Tissue Engineering (Review).去细胞外基质在组织工程中的应用（综述）。
Sovrem Tekhnologii Med. 2022;14(3):57-68. doi: 10.17691/stm2022.14.3.07. Epub 2022 May 28.
6
Discussion on the possibility of multi-layer intelligent technologies to achieve the best recover of musculoskeletal injuries: Smart materials, variable structures, and intelligent therapeutic planning.关于多层智能技术实现肌肉骨骼损伤最佳恢复可能性的探讨：智能材料、可变结构与智能治疗规划
Front Bioeng Biotechnol. 2022 Sep 30;10:1016598. doi: 10.3389/fbioe.2022.1016598. eCollection 2022.
7
Cartilage Formation In Vivo Using High Concentration Collagen-Based Bioink with MSC and Decellularized ECM Granules.体内使用高浓度基于胶原蛋白的生物墨水与 MSC 和脱细胞 ECM 颗粒进行软骨形成。
Int J Mol Sci. 2022 Feb 28;23(5):2703. doi: 10.3390/ijms23052703.
8
A novel gelatin/chitooligosaccharide/demineralized bone matrix composite scaffold and periosteum-derived mesenchymal stem cells for bone tissue engineering.一种用于骨组织工程的新型明胶/壳寡糖/脱矿骨基质复合支架及骨膜来源的间充质干细胞。
Biomater Res. 2021 Jun 16;25(1):19. doi: 10.1186/s40824-021-00220-y.
9
Comparison of the Translational Potential of Human Mesenchymal Progenitor Cells from Different Bone Entities for Autologous 3D Bioprinted Bone Grafts.比较不同骨源间充质祖细胞对自体 3D 生物打印骨移植物的翻译潜能。
Int J Mol Sci. 2021 Jan 14;22(2):796. doi: 10.3390/ijms22020796.
10
Biofabrication of 3D printed hydroxyapatite composite scaffolds for bone regeneration.3D 打印羟基磷灰石复合支架的生物制造用于骨再生。
Biomed Mater. 2021 Mar 8;16(4). doi: 10.1088/1748-605X/abcf03.
人脱矿骨中骨形态发生蛋白-4的含量：与供体年龄及诱导新骨形成能力的关系
J Med Assoc Thai. 2005 Sep;88 Suppl 4:S260-5.
4
Demineralized bone promotes chondrocyte or osteoblast differentiation of human marrow stromal cells cultured in collagen sponges.脱矿骨促进在胶原海绵中培养的人骨髓基质细胞的软骨细胞或成骨细胞分化。
Cell Tissue Bank. 2005;6(1):33-44. doi: 10.1007/s10561-005-4253-y.
5
Extractable bone morphogenetic protein and correlation with induced new bone formation in an in vivo assay in the athymic mouse model.可提取的骨形态发生蛋白及其与无胸腺小鼠模型体内试验中诱导新骨形成的相关性。
Cell Tissue Bank. 2005;6(1):13-23. doi: 10.1007/s10561-005-1445-4.
6
In vitro and in vivo evaluation of differentially demineralized cancellous bone scaffolds combined with human bone marrow stromal cells for tissue engineering.用于组织工程的差异脱矿松质骨支架与人骨髓基质细胞联合的体外和体内评价
Biomaterials. 2005 Jun;26(16):3173-85. doi: 10.1016/j.biomaterials.2004.08.020.
7
Effect of bone extracellular matrix synthesized in vitro on the osteoblastic differentiation of marrow stromal cells.体外合成的骨细胞外基质对骨髓基质细胞成骨分化的影响
Biomaterials. 2005 Mar;26(9):971-7. doi: 10.1016/j.biomaterials.2004.04.001.
8
Controlling mesenchymal stem cell differentiation by TGFBeta family members.通过转化生长因子β家族成员控制间充质干细胞分化
J Orthop Sci. 2003;8(5):740-8. doi: 10.1007/s00776-003-0702-2.
9
Comparison of human bone marrow stromal cells seeded on calcium-deficient hydroxyapatite, beta-tricalcium phosphate and demineralized bone matrix.接种于缺钙羟基磷灰石、β-磷酸三钙和脱矿骨基质上的人骨髓基质细胞的比较。
Biomaterials. 2003 Jul;24(15):2593-603. doi: 10.1016/s0142-9612(03)00062-0.
10
Osteoinduction by demineralised bone.脱矿骨的骨诱导作用。
Int Orthop. 1998;22(5):335-42. doi: 10.1007/s002640050273.