Department of Pharmacology and Biology, College of Life Science and Bioengineering, Beijing University of Technology, P.R. China.
Injury. 2013 Apr;44(4):540-9. doi: 10.1016/j.injury.2012.09.024. Epub 2013 Jan 19.
A potent mesenchymal stem cell (MSC) population was recently isolated from the Wharton's jelly of human umbilical cord (UC). The aim of the current experiments was to determine the potential of human UC-derived MSC (UC-MSC) in cartilage healing.
Chondrogenic differentiation of collagen hydrogel-embedded cells was induced in standard chondrocyte conditioning medium and further detected by real-time PCR, histochemistry and immunohistochemistry analyses. Cell viability and apoptosis of the MSCs in the collagen I hydrogels were monitored using apoptosis detection kit.
Cells isolated from UC were positive for MSC biomarkers and negative for haematopoietic lineage and endothelial biomarkers and possess the capacity to differentiate along osteogenic lineage. UC-MSCs embedded in collagen hydrogel can undergo chondrogenesis characterised by significantly increased expressions of collagen II, aggrecan, COMP (cartilage oligomeric matrix protein) and sox9 after exposed cells-embedded hydrogels to chondrogenic factors. The most of cells remained viable throughout the hydrogels after 3 weeks of cultivation in chondrogenic differentiation medium.
Collagen hydrogel can provide an appropriate 3-D environment for the chondrogenesis of UC-MSCs. UC-MSCs embedded in biocompatible scaffold may have great potential for cartilage engineering.
最近从人脐带华通氏胶中分离出一种具有强大间充质干细胞（MSC）特性的细胞群体。本实验旨在确定人脐带 MSC（UC-MSC）在软骨愈合中的潜在应用。
将细胞包埋在胶原水凝胶中，在标准软骨细胞诱导培养基中诱导其向软骨分化，通过实时 PCR、组织化学和免疫组织化学分析进行进一步检测。通过凋亡检测试剂盒监测胶原 I 水凝胶中 MSC 的细胞活力和凋亡情况。
从 UC 中分离出的细胞 MSC 标志物呈阳性，造血谱系和内皮标志物呈阴性，具有向成骨谱系分化的能力。将 UC-MSC 包埋在胶原水凝胶中，在软骨形成因子的作用下，细胞-包埋水凝胶中的细胞明显增加了 II 型胶原、聚集蛋白聚糖、COMP（软骨寡聚基质蛋白）和 Sox9 的表达，可向软骨分化。在软骨分化培养基中培养 3 周后，大多数细胞在水凝胶中仍然存活。
胶原水凝胶可为 UC-MSC 的软骨形成提供合适的 3D 环境。包埋在生物相容性支架中的 UC-MSC 可能在软骨工程中有巨大的应用潜力。
