Life Science and Food Engineering College, Key Laboratory of Fermentation Resource and Application of Institutes of Higher Learning in Sichuan, Yibin University, Yibin, 644005, People's Republic of China,
Bioprocess Biosyst Eng. 2013 Oct;36(10):1435-43. doi: 10.1007/s00449-013-0887-5. Epub 2013 Jan 22.
It is important to provide added value or to make full use of the co-product of grains from ethanol production. In order to convert distillers' grains into a high-quality feed, the Trichoderma viride and Rhodopseudomonas palustris fermentation were combined and investigated in this study. The T. viride fermentation was carried out in an aerobic fermentation installation in favoring of the growth of the fungi and the degradation of the cellulose, and then the fermentation of R. palustris was performed to increase the content of protein with an anaerobic installation. After the two step fermentations, the true protein content of dried distiller' grains increased from 11.4 to 33.6 % (w/w) (the content of crude protein from 14.5 to 39.7 %), the crude fiber content decreased from 21.3 to 7.6 % (w/w), the crude fat content increased from 5.5 to 7.9 % (w/w), the crude ash decreased from 14.6 to 10.2 % (w/w), the total phosphorus content increased from 0.4 to 1.2 % (w/w), and the water content was 11.8 % (w/w). The dried and fermented grains contain the R. palustris viable count of 5.3 × 10¹¹ CFU/g dry matter. The results may support a new application of an active photosynthetic bacteria fish feed in fisheries industry and offer a reference for the further study of lignocellulosic materials as raw materials converting into high-quality feed.
为了将酒糟转化为高质量的饲料，本研究将绿色木霉和沼泽红假单胞菌发酵相结合。在有氧发酵装置中进行绿色木霉发酵，有利于真菌的生长和纤维素的降解，然后在厌氧装置中进行沼泽红假单胞菌发酵，以增加蛋白质含量。经过两步发酵，干酒糟的真蛋白含量从 11.4%（w/w）增加到 33.6%（w/w）（粗蛋白含量从 14.5%增加到 39.7%），粗纤维含量从 21.3%（w/w）降低到 7.6%（w/w），粗脂肪含量从 5.5%（w/w）增加到 7.9%（w/w），粗灰分含量从 14.6%（w/w）降低到 10.2%（w/w），总磷含量从 0.4%（w/w）增加到 1.2%（w/w），含水量为 11.8%（w/w）。干燥发酵后的酒糟含有 5.3×10¹¹ CFU/g 干物质的沼泽红假单胞菌活菌数。这些结果可能为活性光合细菌鱼饲料在渔业中的新应用提供支持，并为进一步研究木质纤维素材料作为原料转化为高质量饲料提供参考。
