Fishery Machinery and Instrument Research Institute, Chinese Academy of Fisheries Sciences, Shanghai, China.
Microbiologyopen. 2019 Dec;8(12):e924. doi: 10.1002/mbo3.924. Epub 2019 Sep 4.
An approach to culturing attached and suspended forms of Rhodopseudomonas faecalis by using compound fish feed with tap water in transparent containers is reported in this study. The ratio of fish feed to tap water was 14.3-50.8 g/L, and no other inoculum or substances were added during the culture process. When the ratio of fish feed to tap water was 14.3 g/L, the highest total nitrogen, total phosphorus, and total dissolved carbon content recorded in the water in the containers were approximately 730 mg/L, 356 mg/L, and 1,620 mg/L, respectively, during the process of feed decay. Comamonas, Rhodopseudomonas, and Clostridium successively dominated during the culture process. Rhodopseudomonas was the most common dominant genus in both the attached and suspended forms when the water was dark red, and the relative operational taxonomic unit abundance reached 80-89% and 24.8%, respectively. The dominant species was R. faecalis. The maximum thickness of attached bacteria and the biomass of attached Rhodopseudomonas reached up to 0.56 mm and 7.5 mg/cm , respectively. This study provides a method for the mass culture of Rhodopseudomonas by using the fermentation of aquatic compound fish feed.
本研究报告了一种在透明容器中使用复合鱼饲料和自来水培养附着和悬浮形式的粪产碱杆菌的方法。鱼饲料与自来水的比例为 14.3-50.8 g/L，培养过程中未添加其他接种物或物质。当鱼饲料与自来水的比例为 14.3 g/L 时，在饲料降解过程中，容器中水中记录的总氮、总磷和总溶解碳的最高含量分别约为 730 mg/L、356 mg/L 和 1620 mg/L。在培养过程中，交替单胞菌、红假单胞菌和梭菌依次占主导地位。当水呈暗红色时，附着和悬浮形式中最常见的优势属均为红假单胞菌，相对操作分类单元丰度分别达到 80-89%和 24.8%。优势种为粪产碱杆菌。附着细菌的最大厚度和附着红假单胞菌的生物量分别达到 0.56 毫米和 7.5 毫克/平方厘米。本研究提供了一种利用水产复合鱼饲料发酵大规模培养红假单胞菌的方法。
