Department of Veterinary Hygiene and Public Health, School of Veterinary Medicine and Animal Science, UNESP - Univ. Estadual Paulista, Botucatu, State of Sao Paulo, Brazil.
Am J Trop Med Hyg. 2013 Mar;88(3):596-600. doi: 10.4269/ajtmh.12-0332. Epub 2013 Jan 21.
An uncommon disseminated Mycobacterium tuberculosis infection is described in a 12-year-old female dog presenting with fever, dyspnea, cough, weight loss, lymphadenopathy, melena, epistaxis, and emesis. The dog had a history of close contact with its owner, who died of pulmonary tuberculosis. Radiographic examination revealed diffuse radio-opaque images in both lung lobes, diffuse visible masses in abdominal organs, and hilar and mesenteric lymphadenopathy. Bronchial washing samples and feces were negative for acid-fast organisms. Polymerase chain reaction (PCR)-based species identification of bronchial washing samples, feces, and urine revealed M. tuberculosis using PCR-restriction enzyme pattern analysis-PRA. Because of public health concerns, which were worsened by the physical condition of the dog, euthanasia of the animal was recommended. Rough and tough colonies suggestive of M. tuberculosis were observed after microbiological culture of lung, liver, spleen, heart, and lymph node fragments in Löwenstein-Jensen and Stonebrink media. The PRA analysis enabled diagnosis of M. tuberculosis strains isolated from organs.
一只 12 岁雌性犬，表现为发热、呼吸困难、咳嗽、体重减轻、淋巴结病、黑便、鼻出血和呕吐。该犬有与患有肺结核的主人密切接触的病史。放射学检查显示两肺弥漫性不透射线图像、腹部器官弥漫性可见肿块、肺门和肠系膜淋巴结病。支气管灌洗样本和粪便的抗酸杆菌均为阴性。基于聚合酶链反应（PCR）的支气管灌洗样本、粪便和尿液的种属鉴定，使用聚合酶链反应-限制性内切酶图谱分析- PRA 显示分枝杆菌结核。由于公共卫生问题以及犬的身体状况恶化，建议对该动物实施安乐死。在 Löwenstein-Jensen 和 Stonebrink 培养基中对肺、肝、脾、心和淋巴结碎片进行微生物培养后，观察到粗糙坚韧的菌落，提示为结核分枝杆菌。PRA 分析能够诊断从器官分离的结核分枝杆菌株。
