Pan Hui Peng, Chu Dong, Liu Bai Ming, Xie Wen, Wang Shao Li, Wu Qing Jun, Xu Bao Yun, Zhang You Jun
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Environ Entomol. 2013 Feb;42(1):74-8. doi: 10.1603/EN12114.
The impact of symbionts on their hosts depends on their infection density. In the current study, we investigated the effects of host plant and insecticide resistance on the relative amount of symbionts Portiera, Hamiltonella, Rickettsia, and Cardinium in the whitefly Bemisia tabaci (Gennadius) B biotype. The relative amount of symbionts in three host plant-adapted subpopulations (cucumber, Cucumis sativus L.; cabbage, Brassica oleracea L.; and cotton, Gossypium herbaceum L.) with the same genetic background and insecticide (thiamethoxam)-resistant and -susceptible subpopulations with the same genetic background were measured by quantitative polymerase chain reaction. The results showed that the cucumber population harbored more Portiera than the cabbage and cotton populations, the cabbage population harbored more Hamiltonella than the cucumber population, Hamiltonella amount did not statistically differ between the cotton and cucumber or the cotton and cabbage populations, and the cabbage population harbored more Rickettsia and Cardinium than the cucumber and cotton populations. In addition, the thiamethoxam-susceptible population harbored more Portiera and Hamiltonella than the thiamethoxam-resistant population, whereas the thiamethoxam-resistant population harbored more Rickettsia than the thiamethoxam-susceptible population. These results indicated that relative amounts of symbionts were affected significantly by host plant-adaption and insecticide resistance, and the response to host plant and insecticide differed among the symbionts.
共生体对其宿主的影响取决于它们的感染密度。在本研究中，我们调查了宿主植物和杀虫剂抗性对烟粉虱B型生物型（Bemisia tabaci (Gennadius) B biotype）中共生体Portiera、Hamiltonella、立克次氏体（Rickettsia）和卡丁氏菌（Cardinium）相对含量的影响。通过定量聚合酶链反应测定了具有相同遗传背景的三个宿主植物适应亚种群（黄瓜，Cucumis sativus L.；卷心菜，Brassica oleracea L.；棉花，Gossypium herbaceum L.）以及具有相同遗传背景的抗杀虫剂（噻虫嗪）和敏感亚种群中共生体的相对含量。结果表明，黄瓜种群中Portiera的含量高于卷心菜和棉花种群，卷心菜种群中Hamiltonella的含量高于黄瓜种群，棉花和黄瓜种群或棉花和卷心菜种群之间Hamiltonella的含量在统计学上无差异，卷心菜种群中立克次氏体和卡丁氏菌的含量高于黄瓜和棉花种群。此外，噻虫嗪敏感种群中Portiera和Hamiltonella的含量高于噻虫嗪抗性种群，而噻虫嗪抗性种群中立克次氏体的含量高于噻虫嗪敏感种群。这些结果表明，共生体的相对含量受宿主植物适应性和杀虫剂抗性的显著影响，并且不同共生体对宿主植物和杀虫剂的反应存在差异。
