Key Laboratory of Biogeography and Bioresource in Arid Land, Chinese Academy of Science, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Ürűmqi, China.
J Antibiot (Tokyo). 2013 Apr;66(4):205-9. doi: 10.1038/ja.2012.119. Epub 2013 Jan 23.
A novel actinobacterium MBRL 251(T), isolated from a sample collected from a limestone quarry at Hundung, Manipur, India, was characterized using a polyphasic approach. The 16S ribosomal RNA gene sequence of strain MBRL 251(T) showed closest similarities with Streptomyces xanthochromogenes NRRL B-5410(T) (99.6%) and Streptomyces michiganensis NBRC 12797(T) (99.6%). The DNA relatedness between MBRL 251(T) and S. xanthochromogenes NBRC 12828(T), and S. michiganensis NBRC 12797(T) was 46.6% and 40.7%, respectively. Strain MBRL 251(T) contained LL-diaminopimelic acid as the diagnostic diamino acid, with glucose and xylose as the main cell wall sugars, whereas small amounts of galactose, mannose, rhamnose and ribose were also detected in the whole-cell wall hydrolysates. The major fatty acids identified were anteiso-C15:0 (35.1%), iso-C16:0 (21.1%) and anteiso-C17:1 (13.2%). The predominant menaquinones detected were MK-9(H6) and MK-9(H8), whereas the polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositolmannosides. The G+C content of the genomic DNA was 72.3%. The phenotypic and genotypic data showed that strain MBRL 251(T) merits the recognition as a representative of a novel species of the genus Streptomyces. It is proposed that the isolate should be classified in the genus Streptomyces as a novel species, Streptomyces hundungensis sp. nov. The type strain is MBRL 251(T) (=JCM 17577(T)=KCTC 29124(T)).
一株新的放线菌 MBRL 251(T)，从印度曼尼普尔 Hundung 的一个石灰岩采石场采集的样本中分离出来，通过多相鉴定法进行了鉴定。菌株 MBRL 251(T)的 16S 核糖体 RNA 基因序列与黄灰链霉菌 NRRL B-5410(T)（99.6%）和密歇根链霉菌 NBRC 12797(T)（99.6%）最为相似。MBRL 251(T)与黄灰链霉菌 NBRC 12828(T)和密歇根链霉菌 NBRC 12797(T)的 DNA 相关性分别为 46.6%和 40.7%。菌株 MBRL 251(T)含有 LL-二氨基庚二酸作为诊断二氨基庚二酸，细胞壁糖主要为葡萄糖和木糖，而全细胞壁水解物中也检测到少量的半乳糖、甘露糖、鼠李糖和核糖。鉴定出的主要脂肪酸为anteiso-C15:0（35.1%）、iso-C16:0（21.1%）和anteiso-C17:1（13.2%）。检测到的主要menaquinones 为 MK-9(H6)和 MK-9(H8)，而极性脂类为双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇和磷脂酰肌醇甘露糖苷。基因组 DNA 的 G+C 含量为 72.3%。表型和基因型数据表明，菌株 MBRL 251(T)是链霉菌属的一个新种的代表，值得认可。建议将该分离物归类为链霉菌属的一个新种，即 Hundungensis 链霉菌。模式菌株为 MBRL 251(T)=JCM 17577(T)=KCTC 29124(T)。
