Lorentz K, Weiss T
J Clin Chem Clin Biochem. 1986 May;24(5):309-23.
Seven methods for protein determination in urine were systematically checked, viz. turbidimetric assay with benzethonium chloride, turbidimetry with trichloroacetic acid of various concentrations, micellar complex formation with Coomassie Brilliant Blue G-250, formation and gel filtration of protein complexes with copper, reaction of protein tannin compounds with iron, and biuret reaction after different precipitation procedures. The evaluation was mainly based on the following criteria: identical reaction with various proteins, stable and linear absorbance with concentration, complete recovery, and greatest possible freedom from interferences. On this basis, the biuret reaction must now be regarded as the method of choice. Albumin and gamma-globulins are reliably estimated after precipitation by perchloric acid or by an ethanol-diethylether mixture, whereas all proteins, including uromucoid, may be measured after precipitation by Tsuchiya's reagent. Interference from urinary pigments can be avoided by reading versus a sample blank.
系统地检验了七种尿液蛋白质测定方法，即：用苄索氯铵进行比浊法测定、用不同浓度的三氯乙酸进行比浊法测定、与考马斯亮蓝G - 250形成胶束复合物、与铜形成蛋白质复合物并进行凝胶过滤、蛋白质单宁化合物与铁的反应以及不同沉淀程序后的双缩脲反应。评估主要基于以下标准：与各种蛋白质反应相同、吸光度随浓度稳定且呈线性、回收率完全以及尽可能不受干扰。在此基础上，现在必须将双缩脲反应视为首选方法。用高氯酸或乙醇 - 乙醚混合物沉淀后可可靠地估计白蛋白和γ - 球蛋白，而包括尿黏蛋白在内的所有蛋白质在用土屋试剂沉淀后均可进行测定。通过与样品空白对照读数可避免尿色素的干扰。
