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电压依赖性阴离子通道1(AtVDAC1)在拟南芥种子萌发和幼苗发育过程中对植物的低温反应起负调控作用,并与钙传感器CBL1相互作用。

The voltage-dependent anion channel 1 (AtVDAC1) negatively regulates plant cold responses during germination and seedling development in Arabidopsis and interacts with calcium sensor CBL1.

作者信息

Li Zhi-Yong, Xu Zhao-Shi, He Guang-Yuan, Yang Guang-Xiao, Chen Ming, Li Lian-Cheng, Ma Youzhi

机构信息

The Genetic Engineering International Cooperation Base of Chinese Ministry of Science and Technology, Chinese National Center of Plant Gene Research (Wuhan) HUST Part, College of Life Science and Technology, Huazhong University of Science & Technology (HUST), Wuhan 430074, China.

出版信息

Int J Mol Sci. 2013 Jan 4;14(1):701-13. doi: 10.3390/ijms14010701.

Abstract

The voltage-dependent anion channel (VDAC), a highly conserved major mitochondrial outer membrane protein, plays crucial roles in energy metabolism and metabolite transport. However, knowledge about the roles of the VDAC family in plants is limited. In this study, we investigated the expression pattern of VDAC1 in Arabidopsis and found that cold stress promoted the accumulation of VDAC1 transcripts in imbibed seeds and mature plants. Overexpression of VDAC1 reduced tolerance to cold stress in Arabidopsis. Phenotype analysis of VDAC1 T-DNA insertion mutant plants indicated that a vdac1 mutant line had faster germination kinetics under cold treatment and showed enhanced tolerance to freezing. The yeast two-hybrid system revealed that VDAC1 interacts with CBL1, a calcium sensor in plants. Like the vdac1, a cbl1 mutant also exhibited a higher seed germination rate. We conclude that both VDAC1 and CBL1 regulate cold stress responses during seed germination and plant development.

摘要

电压依赖性阴离子通道(VDAC)是一种高度保守的主要线粒体外膜蛋白,在能量代谢和代谢物运输中起关键作用。然而,关于VDAC家族在植物中的作用的了解有限。在本研究中,我们研究了拟南芥中VDAC1的表达模式,发现冷胁迫促进了VDAC1转录本在吸胀种子和成熟植株中的积累。VDAC1的过表达降低了拟南芥对冷胁迫的耐受性。VDAC1 T-DNA插入突变体植株的表型分析表明,一个vdac1突变株系在冷处理下具有更快的萌发动力学,并且对冷冻表现出增强的耐受性。酵母双杂交系统显示VDAC1与植物中的钙传感器CBL1相互作用。与vdac1一样,cbl1突变体也表现出较高的种子萌发率。我们得出结论,VDAC1和CBL1在种子萌发和植物发育过程中均调节冷胁迫反应。

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