Brazhe Ulyanova Nadiya A, Erokhova Liudmila A, Churin Anatolii A, Maksimov Georgy V
Department of Biophysics, Biological Faculty, Moscow State University, Leninskie gory, 1/12, Moscow, Russia 119992.
J Biol Phys. 2005 Dec;31(3-4):533-46. doi: 10.1007/s10867-005-2043-1.
Rhythmic activity, synaptic transmission and propagation of excitation depend on the processes that occur at different cellular levels. Modulation of neuron activity results from the changes of plasma membrane and organelles properties. We aimed to study the relationship between changes of the ion channels activity, membrane microviscosity and amount of bound Ca(2+) under the influence of nitric oxide (NO). We also investigated the effect of NO on the refractive index of neurons. We have shown that NO activates voltage-dependent K(K(V))-channels and leads to the decrease in the amount of membrane-bound and stored Ca(2+). NO causes a decrease in the microviscosity of the membrane of cytosomes and altered refractive index of neurones. The latter can result from the modification of plasma membrane physico-chemical properties and structural changes in the cytoplasm (vesicles movement, reorganisation of cytoskeleton, etc.). It is suggested that long-term modifications of the physico-chemical properties of membranes and reorganisation in the cytoplasm affect neuron activity and signal transmission.
节律性活动、突触传递和兴奋的传播取决于在不同细胞水平上发生的过程。神经元活动的调节源于质膜和细胞器特性的变化。我们旨在研究一氧化氮（NO）影响下离子通道活性、膜微粘度和结合钙（Ca²⁺）量的变化之间的关系。我们还研究了NO对神经元折射率的影响。我们已经表明，NO激活电压依赖性钾通道（K(V)），并导致膜结合和储存的Ca²⁺量减少。NO导致细胞体膜的微粘度降低，并改变神经元的折射率。后者可能是由于质膜物理化学性质的改变和细胞质中的结构变化（囊泡运动、细胞骨架重组等）引起的。有人认为，膜物理化学性质的长期改变和细胞质中的重组会影响神经元活动和信号传递。
