Kotsyuba Elena P, Vaschenko Marina A
A. V. Zhirmunsky Institute of Marine Biology, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, 690041, Russia.
Invert Neurosci. 2010 Nov;10(1):35-46. doi: 10.1007/s10158-010-0103-0. Epub 2010 May 26.
We studied here neuron ultrastructure, synaptic plasticity and subcellular localization of NADPH-diaphorase (NADPH-d), a cytochemical marker for nitric oxide syntase, in the pedal ganglia of the Gray mussel Crenomytilus grayanus sampled from the polluted and reference sites in Amursky Bay (Sea of Japan) at lower and higher water temperature (in the beginning and the end of August, respectively). At lower temperature, neuroplastic changes in mussel ganglia prevailed: a sharp increase in the number of cytosomes in NADPH-d-positive neurons and a sharp decrease in the number of mitochondria in both NADPH-d-positive and NADPH-d-negative neurons. At higher temperature, neurodegenerative changes prevailed: disruption of a part of NADPH-d-negative axons and interneuronal contacts, formation of concentric lamellar structures in the neuropils, and accumulation of autophagosomes in NADPH-d negative neurons. The results suggest that the stress-induced production of nitric oxide in cytosomes of mussel neurons and plasticity of gap junctions have a neuroprotective effect.
我们在此研究了采自日本海阿穆尔湾污染区和对照区的灰贻贝（Crenomytilus grayanus）足神经节中神经元的超微结构、突触可塑性以及一氧化氮合酶的细胞化学标志物烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）的亚细胞定位，实验分别在较低和较高水温下进行（分别于8月初和8月末）。在较低温度下，贻贝神经节中以神经可塑性变化为主：NADPH-d阳性神经元中胞质体数量急剧增加，NADPH-d阳性和阴性神经元中线粒体数量急剧减少。在较高温度下，以神经退行性变化为主：部分NADPH-d阴性轴突和神经元间接触遭到破坏，神经纤维网中形成同心层状结构，NADPH-d阴性神经元中自噬体积累。结果表明，贻贝神经元胞质体中应激诱导产生的一氧化氮以及缝隙连接的可塑性具有神经保护作用。
