Department of Food and Nutrition, Eulji University, Seongnam, Gyunggi 461-723, Korea.
Nutr Res Pract. 2012 Dec;6(6):499-504. doi: 10.4162/nrp.2012.6.6.499. Epub 2012 Dec 31.
This study attempted to investigate the effects of resveratrol on the differentiation of adipocytes. After cells were treated with various concentrations of resveratrol (0, 10, 20, and 40 µmol/L), adipocyte proliferation, the protein expression of transcription factors, and MMPs' activities were determined. Cell proliferation was inhibited more within 4 days of incubation (P < 0.05), and lipid accumulation in adipocyte was significantly inhibited by 93.8%, 92.4% and 91.5%, respectively, after two days of 10, 20, and 40 µmol/L resveratrol treatment (P < 0.05). Six days of incubation with the three resveratrol concentrations caused a significantly decreases of 63%, 59.9%, and 25.1% GPDH activity as a dose-dependent response. The triglyceride concentration also decreased significantly with the increase of resveratrol concentration (P < 0.05). The protein expression of CCAAT/enhancer-binding protein (C/EBPβ) was decreased significantly by 56% and 30% while PPARγ was significantly reduced by 57% and 15% with resveratrol treatments of 20 and 40 µmol/L, respectively (P < 0.05). The protein expression of C/EBPα was decreased by 83%, 74%, and 38% to increased dosage levels, with significance determined for this decrease from 20 µmol/L of resveratrol. The protein expression of fatty acid binding protein (FABP4) was decreased significantly by 88%, 72%, and 46% with the increase of resveratrol concentration. The activity of MMP-2 was decreased significantly by 84%, 70%, and 63% while MMP-9 activity was decreased significantly by 74%, 62%, and 39% with the increased resveratrol concentrations of 10, 20, and 40 µmol/L, respectively (P < 0.05).
本研究旨在探讨白藜芦醇对脂肪细胞分化的影响。将细胞分别用不同浓度的白藜芦醇（0、10、20 和 40μmol/L）处理后，测定细胞增殖、转录因子的蛋白表达和 MMPs 的活性。结果发现，细胞在孵育的第 4 天增殖受到更显著的抑制（P＜0.05），在经过 10、20 和 40μmol/L 白藜芦醇处理 2 天后，脂肪细胞中的脂质积累分别显著抑制了 93.8%、92.4%和 91.5%（P＜0.05）。孵育 6 天后，三种白藜芦醇浓度下，GPDH 活性均呈剂量依赖性显著降低，分别为 63%、59.9%和 25.1%。随着白藜芦醇浓度的增加，甘油三酯浓度也显著降低（P＜0.05）。蛋白表达水平显示，C/EBPβ的表达显著降低了 56%和 30%，而 PPARγ则分别降低了 57%和 15%，两种情况在 20μmol/L 和 40μmol/L 的白藜芦醇处理中均具有统计学意义（P＜0.05）。C/EBPα的蛋白表达水平随着剂量的增加而降低，降低幅度分别为 83%、74%和 38%，20μmol/L 白藜芦醇处理时差异具有统计学意义。脂肪酸结合蛋白（FABP4）的蛋白表达水平随着白藜芦醇浓度的增加而显著降低，降低幅度分别为 88%、72%和 46%。随着白藜芦醇浓度的增加，MMP-2 的活性分别显著降低了 84%、70%和 63%，MMP-9 的活性分别显著降低了 74%、62%和 39%，在 10、20 和 40μmol/L 的白藜芦醇浓度下分别具有统计学意义（P＜0.05）。
