Department of Mechanical Engineering, Istanbul Technical University, Istanbul, Turkey.
Electrophoresis. 2013 Apr;34(7):1051-8. doi: 10.1002/elps.201200411. Epub 2013 Mar 7.
In this study, a separability parameter is introduced to determine the selection of optimum operating parameters for DEP separation of a cell pair. The separability parameter is defined as a function of cells' Clausius-Mossotti (CM) factors. T-cell leukemia Jurkat and mouse melanoma B16 cells are tested to validate the separability parameter. CM factors of cells are measured using a recently developed microfluidic impedance spectroscopy device. Separability maps are generated for varying values of field frequency and buffer conductivity. Cell separation is tested using a planar interdigitated electrode array at different buffer conductivities. Impedance measurements of the DEP device are performed at various buffer conductivities. Electrode polarization effects and energy allocation for dielectrophoretic manipulation of cells are computed from the impedance data utilizing an equivalent circuit model. Cell separation results are explained in the light of the impedance measurements.
在这项研究中，引入了可分离性参数来确定 DEP 分离细胞对的最佳操作参数的选择。该可分离性参数定义为细胞 Clausius-Mossotti（CM）因子的函数。使用最近开发的微流控阻抗谱设备测量 T 细胞白血病 Jurkat 和小鼠黑色素瘤 B16 细胞的 CM 因子。针对不同的场频率和缓冲液电导率值生成可分离性图谱。在不同的缓冲液电导率下使用平面叉指电极阵列测试细胞分离。在不同的缓冲液电导率下进行 DEP 装置的阻抗测量。利用等效电路模型从阻抗数据计算电极极化效应和用于细胞介电泳操作的能量分配。根据阻抗测量结果解释细胞分离结果。
