Chandrasekhar Veeramachaneni, Amulya Vanapatla, Rani Vanapatla Swaroopa, Prakash T Jaya, Ranjani A Siva, Gayathri Ch
Department of Conservative Dentistry and Endodontics, Mamata Dental College, Khammam, Andhra Pradesh, India.
J Conserv Dent. 2013 Jan;16(1):36-40. doi: 10.4103/0972-0707.105296.
To evaluate the biocompatibility of a new root canal irrigant Q mix™ 2 in 1 in comparison to 0.9% sterile saline, 3% sodium hypochlorite (NaOCl), 2% chlorhexidine (CHX), and 17% ethylenediaminetetraacetic acid (EDTA).
Six circles were drawn on the dorsal skin of 24 male Wistar Albino rats, leaving 2cm between each circle. Using a syringe, 0.1mL of each root canal irrigant was injected subcutaneously into five circles. In the sixth circle, the needle of an empty syringe was introduced into the skin, but no irrigant was injected (control group). Evaluations were done at 2 hours, 48 hours, 14 days, and 30 days postprocedure. Tissue samples were excised, embedded in paraffin blocks, and 3 μm thick sections were obtained and stained with hematoxylin and eosin. The areas of inflammatory reaction were evaluated. From each tissue sample, five sections presenting the greatest inflammatory reactions were examined under a light microscope, and analyzed statistically by analysis of variance (ANOVA) and Tukey's test.
At the two-hour examination period, all the irrigants showed a slight increase in the number of inflammatory cells, at 48 hours, the number of inflammatory cells were increased significantly, and after 14 and 30 days, they were decreased gradually. Qmix™ 2 in 1 showed a smaller number of inflammatory cells than other irrigants tested.
QMix™ 2 in 1 was shown to be less toxic to the rat subcutaneous tissue than 3% NaOCl, 2% CHX, and 17% EDTA.
评估新型根管冲洗剂Q mix™ 2合1与0.9%无菌生理盐水、3%次氯酸钠（NaOCl）、2%氯己定（CHX）和17%乙二胺四乙酸（EDTA）相比的生物相容性。
在24只雄性Wistar白化大鼠的背部皮肤绘制6个圆圈，每个圆圈之间间隔2cm。使用注射器将0.1mL每种根管冲洗剂皮下注射到5个圆圈中。在第6个圆圈中，将空注射器的针头刺入皮肤，但不注射冲洗剂（对照组）。在操作后2小时、48小时、14天和30天进行评估。切除组织样本，嵌入石蜡块，获得3μm厚的切片，并用苏木精和伊红染色。评估炎症反应区域。从每个组织样本中选取5个炎症反应最明显的切片，在光学显微镜下检查，并通过方差分析（ANOVA）和Tukey检验进行统计学分析。
在两小时的检查期，所有冲洗剂的炎症细胞数量均略有增加，在48小时时，炎症细胞数量显著增加，在14天和30天后逐渐减少。Q mix™ 2合1的炎症细胞数量比其他测试冲洗剂少。
与3% NaOCl、2% CHX和17% EDTA相比，QMix™ 2合1对大鼠皮下组织的毒性较小。
