Department of Imaging System Engineering, Pukyong National University, Busan 608-737, Republic of Korea.
J Colloid Interface Sci. 2013 Mar 15;394:132-40. doi: 10.1016/j.jcis.2012.11.068. Epub 2013 Jan 5.
Hydroxyapatite nanocrystals (HAP NCs) were encapsulated by poly(allyl methacrylate) (PolyAMA) employing controlled surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) polymerization of allyl methacrylate to afford HAP-PolyAMA nanohybrids. The subsequent thiol-ene coupling of nanohybrids with 2-mercaptosuccinic acid resulted HAP-Poly(AMA-COOH) possessing multicarboxyl group. The formation of the nanohybrids was confirmed by FT-IR and EDS analyses. The TGA and FE-SEM investigation were further suggested the grafting of PolyAMA onto HAP NCs. The utility of the HAP-PolyAMA nanohybrid as drug carrier was also explored. The pendant carboxyl groups on the external layers of nanohybrids were conjugated with anticancer drug cisplatin to afford HAP-Poly(AMA-COOH)/Pt complex. The formation of the complex was confirmed by FT-IR, XPS, and FE-SEM. In vitro evaluation of the synthesized complex as nanomedicine revealed its potential chemotherapeutic efficacy against cancer cell lines.
羟基磷灰石纳米晶（HAP NCs）被聚甲基丙烯酰胺（PolyAMA）包裹，采用可控的表面引发可逆加成-断裂链转移（SI-RAFT）聚合甲基丙烯酰胺，得到 HAP-PolyAMA 纳米杂化材料。随后纳米杂化物与 2-巯基琥珀酸的硫醇-烯加成反应得到具有多个羧基的 HAP-Poly（AMA-COOH）。FT-IR 和 EDS 分析证实了纳米杂化物的形成。TGA 和 FE-SEM 研究进一步表明，PolyAMA 接枝到 HAP NCs 上。还探索了 HAP-PolyAMA 纳米杂化物作为药物载体的用途。纳米杂化物外表面的悬挂羧基与抗癌药物顺铂偶联，得到 HAP-Poly（AMA-COOH）/Pt 配合物。FT-IR、XPS 和 FE-SEM 证实了配合物的形成。作为纳米药物的合成配合物的体外评估显示了其对癌细胞系的潜在化疗功效。
