Centre de Recherche sur les Macromolécules Végétales (CERMAV-CNRS), affiliated to Grenoble-Université and ICMG, Grenoble, France.
Chem Soc Rev. 2013 Jun 7;42(11):4798-813. doi: 10.1039/c2cs35435g. Epub 2013 Jan 25.
The large diversity and complexity of glycan structures together with their crucial role in many biological or pathological processes require the development of new high-throughput techniques for analyses. Lectins are classically used for characterising, imaging or targeting glycoconjugates and, when printed on microarrays, they are very useful tools for profiling glycomes. Development of recombinant lectins gives access to reliable and reproducible material, while engineering of new binding sites on existing scaffolds allows tuning of specificity. From the accumulated knowledge on protein-carbohydrate interactions, it is now possible to use nucleotide and peptide (bio)synthesis for producing new carbohydrate-binding molecules. Such a biomimetic approach can also be addressed by boron chemistry and supra-molecular chemistry for the design of fully artificial glycosensors.
糖链结构的多样性和复杂性，加上它们在许多生物或病理过程中的关键作用，都需要开发新的高通量技术来进行分析。凝集素通常用于糖缀合物的特征描述、成像或靶向，当被打印在微阵列上时，它们是分析糖组的非常有用的工具。重组凝集素的开发可以获得可靠和可重复的材料，而在现有支架上设计新的结合位点则可以调整特异性。从积累的蛋白质-碳水化合物相互作用知识中，现在可以使用核苷酸和肽（生物）合成来生产新的碳水化合物结合分子。通过硼化学和超分子化学设计完全人工的糖传感器也可以解决这种仿生方法。
