Gilbert A M, Stich H F, Rosin M P, Davison A J
Environmental Carcinogenesis Unit, British Columbia Cancer Research Centre, Vancouver, Canada.
Int J Cancer. 1990 May 15;45(5):855-9. doi: 10.1002/ijc.2910450512.
Variations in the response of individuals to beta-carotene supplementation were studied by measuring the accumulation of beta-carotene in oral mucosa cells. Beta-carotene was administered orally to 178 individuals for 3 consecutive days, exfoliated oral mucosa cells were collected by brushing the entire oral mucosa on the 7th day following supplementation, and the beta-carotene content was measured by HPLC analysis of the pronase-treated cells. The rise in beta-carotene levels in the oral mucosa following supplementation varied considerably. Significant differences in mean beta-carotene levels in the oral mucosa were observed in 4 population groups. After supplementation, beta-carotene levels increased by factors of 10.3 (100 Mile House), 7.8 (Williams Lake), 6.9 (Lytton) and 3.4 (Vancouver), respectively. This difference in mean beta-carotene values is due to there being different proportions of weak and strong responders in the various population groups. Neither peak levels nor increases in beta-carotene levels were correlated with base-line concentrations of beta-carotene in the pre-supplementation samples. A second supplementation was given to 54 individuals several months after the initial supplementation. Of 17 weak responders in the first supplementation study, 10 (58.8%) individuals again showed only a small increase. A time-course study revealed that low responders showed no significant changes in beta-carotene levels over the 21 days following supplementation. Particular attention should be paid to weak responders when results of intervention trials using beta-carotene are interpreted.
通过测量β-胡萝卜素在口腔黏膜细胞中的蓄积情况，研究了个体对补充β-胡萝卜素反应的差异。对178名个体连续3天口服β-胡萝卜素，在补充后的第7天，通过刷整个口腔黏膜收集脱落的口腔黏膜细胞，并通过对经链霉蛋白酶处理的细胞进行高效液相色谱分析来测量β-胡萝卜素含量。补充后口腔黏膜中β-胡萝卜素水平的升高差异很大。在4个人群组中观察到口腔黏膜中β-胡萝卜素平均水平存在显著差异。补充后，β-胡萝卜素水平分别增加了10.3倍（100英里屋）、7.8倍（威廉姆斯湖）、6.9倍（利顿）和3.4倍（温哥华）。平均β-胡萝卜素值的这种差异是由于不同人群组中弱反应者和强反应者的比例不同。β-胡萝卜素的峰值水平和水平升高均与补充前样本中β-胡萝卜素的基线浓度无关。在首次补充几个月后，对54名个体进行了第二次补充。在首次补充研究的17名弱反应者中，有10名（58.8%）个体再次仅显示出小幅增加。一项时间进程研究表明，低反应者在补充后的21天内β-胡萝卜素水平没有显著变化。在解释使用β-胡萝卜素的干预试验结果时，应特别关注弱反应者。
