Stich H F, Hornby A P, Dunn B P
Int J Cancer. 1986 Mar 15;37(3):389-93. doi: 10.1002/ijc.2910370310.
Beta-carotene was estimated in exfoliated oral mucosa cells in groups of individuals at various risks for oral cancer. Approximately 4 X 10(6) exfoliated cells were collected from each subject by brushing the oral mucosa. Cell pellets were hydrolyzed with pronase and then with KOH/methanol. Beta-carotene was extracted with hexane, separated by reverse-phase HPLC, and detected at 450 nm. Mean beta-carotene levels in exfoliated cells were 0.08 ng/10(6) cells for 56 heavy consumers of alcoholic beverages (150 g or more per week), 1.36 ng/10(6) cells for 28 Seventh Day Adventists (all abstainers from alcohol, tobacco and meat consumption), 1.39 ng/10(6) cells for 55 lacto-vegetarians of the International Society for Krishna Consciousness (ISKC) (abstainers from alcohol and tobacco), and 1.08 ng/10(6) cells for 61 representatives of a "Western" life-style pattern (64% consumed the equivalent of at least one bottle of wine or 7 bottles of beer per week, and all were non-smokers). If the heavy alcohol consumers (males) are matched to non-drinking males of comparable age, the mean beta-carotene values are 0.08 ng versus 1.24 ng/10(6) cells. The possible involvement of the low levels of beta-carotene in the mucosa of heavy alcohol drinkers in increased sensitivity towards the carcinogenic and genotoxic activity of cigarette smoking plus alcohol ingestion is discussed.
在处于不同口腔癌风险的个体组中，对脱落口腔黏膜细胞中的β-胡萝卜素进行了评估。通过刷取口腔黏膜，从每个受试者收集约4×10⁶个脱落细胞。细胞沉淀先用链霉蛋白酶水解，然后用氢氧化钾/甲醇水解。β-胡萝卜素用己烷提取，通过反相高效液相色谱分离，并在450nm处检测。对于56名重度酒精饮料消费者（每周饮用150克或更多），脱落细胞中的β-胡萝卜素平均水平为0.08纳克/10⁶个细胞；对于28名基督复临安息日会信徒（均戒酒、戒烟且不食用肉类），为1.36纳克/10⁶个细胞；对于国际奎师那知觉协会（ISKC）的55名乳素食者（戒酒、戒烟），为1.39纳克/10⁶个细胞；对于61名具有“西方”生活方式模式的代表（64%的人每周饮用相当于至少一瓶葡萄酒或7瓶啤酒，且均不吸烟），为1.08纳克/10⁶个细胞。如果将重度酒精消费者（男性）与年龄相当的非饮酒男性进行匹配，β-胡萝卜素的平均含量为0.08纳克/10⁶个细胞，而对照组为1.24纳克/10⁶个细胞。文中讨论了重度饮酒者黏膜中β-胡萝卜素水平较低可能与对吸烟加饮酒的致癌和基因毒性活性敏感性增加有关。
