Division of Neonatology, Department of Pediatrics, Faculty of Medicine, Prince of Songkla University, 15 Kanjanavanit Road, Tambon Korhong, Hat Yai District, Songkhla, 90110, Thailand,
Indian J Pediatr. 2013 Oct;80(10):809-13. doi: 10.1007/s12098-013-0963-5. Epub 2013 Jan 26.
To compare the fat content and contamination of expressed breast milk (EBM) before and after storage (30 d) in hard polypropylene containers (HC) and soft polyethylene bags (SB) containers.
Ninety specimens of EBM were collected into HC and separated into two HC and two SB. The fat content of each specimen of EBM in HC and SB was measured and cultures were performed. The specimens in the second HC and SB containers were kept frozen for 30 d before thawing and then measuring the fat content and performing cultures.
The means ± SD of the fat content of fresh and thawed EBM in HC were 2.98 ± 0.97 and 2.66 ± 0.88 g/100 ml, respectively, with a loss of 0.32 g/100 ml (p < 0.001). The means ± SD of the fat content of fresh and thawed EBM in SB were 3.06 ± 1.00 and 2.77 ± 0.91 g/100 ml, respectively, with a mean loss of 0.29 g/100 ml during storage (p < 0.001). The loss of fat content during frozen storage did not differ significantly between the two types of containers (p = 0.53). All bacterial cultures of fresh and thawed EBM in HC and SB showed only nonpathogenic organisms.
SB can replace HC for the long-term storage of frozen EBM of up to 30 d without deleterious effects on fat loss or contamination.
比较硬聚丙烯容器(HC)和软聚乙烯袋(SB)储存前和储存 30 天后母乳(EBM)的脂肪含量和污染情况。
收集 90 份 EBM 标本,分别放入 HC 和 SB 中,分为 2 组 HC 和 2 组 SB。测量每组 HC 和 SB 中 EBM 的脂肪含量,并进行培养。将第二组 HC 和 SB 容器中的标本冷冻保存 30 天,然后解冻并测量脂肪含量和进行培养。
HC 中新鲜和解冻 EBM 的脂肪含量均值±标准差分别为 2.98±0.97 和 2.66±0.88 g/100 ml,分别损失了 0.32 g/100 ml(p<0.001)。SB 中新鲜和解冻 EBM 的脂肪含量均值±标准差分别为 3.06±1.00 和 2.77±0.91 g/100 ml,分别损失了 0.29 g/100 ml(p<0.001)。两种容器储存过程中脂肪含量的损失没有显著差异(p=0.53)。HC 和 SB 中新鲜和解冻 EBM 的所有细菌培养均显示仅为非致病性生物。
SB 可替代 HC 用于冷冻储存 EBM,最长储存 30 天,不会对脂肪损失或污染产生有害影响。